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Gene Review

CDC14  -  phosphoprotein phosphatase CDC14

Saccharomyces cerevisiae S288c

Synonyms: OAF3, Tyrosine-protein phosphatase CDC14, YFR028C
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High impact information on CDC14


Biological context of CDC14


Anatomical context of CDC14


Associations of CDC14 with chemical compounds

  • We report here that temperature-sensitive (ts) dhf2-2 mutant cells can be rescued by overexpression of CDC14, which encodes a dual-specificity protein phosphatase, when grown on glucose-containing medium, as reported previously, but not on galactose [11].
  • CDC14 encodes a phosphotyrosine phosphatase (PTPase) and is essential for termination of M phase [12].
  • Screening of two S. cerevisiae cDNA libraries led to the identification of CBT1 as a gene which, if overexpressed simultaneously with CDC14, results in the rescue of the dbJ2-2 mutation at restrictive temperature on galactose-based medium, as well as on media containing non-fermentable carbon sources such as glycerol, lactate and acetate [11].
  • The essential CDC14 gene of the budding yeast, Saccharomyces cerevisiae, encodes a 62-kDa protein containing a sequence that conforms to the active site motif found in all enzymes of the protein tyrosine phosphatase superfamily [13].
  • However, unlike histone mRNA, which is repressed upon hydroxyurea arrest, CDC14 mRNA appears to be unaffected [14].

Physical interactions of CDC14


Enzymatic interactions of CDC14

  • Cdc14 activates the degradation of Clb cyclins by dephosphorylating the APC-specificity factor Cdh1 [18].
  • Cdc14p mediates mitotic exit by dephosphorylating Cdk1p substrates and promoting Cdk1p inactivation [19].
  • Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles [20].

Regulatory relationships of CDC14

  • An extra copy of CDC14 suppressed the temperature sensitivity of cdc15-rlt1 cells, but not that of cdc15-1 cells [12].
  • The highly conserved protein phosphatase Cdc14 promotes both Clb degradation and Sic1 accumulation [18].
  • The level of the CDC14 transcript appears to be weakly cell cycle-regulated and has a periodicity which lags approximately 15 min behind histone HTB1 mRNA accumulation levels [14].
  • This delocalization of Cdc14 was suppressed by the deletion of SPO12 [21].
  • From G1 through anaphase, RENT localizes to the nucleolus, and Cdc14 activity is inhibited by Net1 [22].

Other interactions of CDC14

  • However, in cdc6 and cdc14 mutants, the mitotic loss of pDK243 with an ARS cluster was suppressed by a factor of 6-8 compared to pDK243 without the cluster [3].
  • But how the MEN residing at the spindle pole body (SPB) controls the association of Cdc14 with Net1/Cfi1 in the nucleolus is not yet understood [7].
  • We conclude that Cdc14 is released from the nucleolus at the onset of anaphase in a CDC5-dependent manner and that MEN factors possibly regulate Cdc14 release from the SPB [7].
  • We provide evidence suggesting that Cdc14p is the phosphatase responsible for the dephosphorylation of Cdc15p [23].
  • The SPB localization of Cdc14 was significantly affected by the MEN mutations and the bub2 mutation [7].

Analytical, diagnostic and therapeutic context of CDC14


  1. Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast. Queralt, E., Lehane, C., Novak, B., Uhlmann, F. Cell (2006) [Pubmed]
  2. Cdc14 phosphatase induces rDNA condensation and resolves cohesin-independent cohesion during budding yeast anaphase. Sullivan, M., Higuchi, T., Katis, V.L., Uhlmann, F. Cell (2004) [Pubmed]
  3. Addition of extra origins of replication to a minichromosome suppresses its mitotic loss in cdc6 and cdc14 mutants of Saccharomyces cerevisiae. Hogan, E., Koshland, D. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  4. Mutations in CDC14 result in high sensitivity to cyclin gene dosage in Saccharomyces cerevisiae. Yuste-Rojas, M., Cross, F.R. Mol. Gen. Genet. (2000) [Pubmed]
  5. The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae. Grandin, N., de Almeida, A., Charbonneau, M. Mol. Gen. Genet. (1998) [Pubmed]
  6. A single-copy suppressor of the Saccharomyces cerevisae late-mitotic mutants cdc15 and dbf2 is encoded by the Candida albicans CDC14 gene. Jiménez, J., Cid, V.J., Nombela, C., Sánchez, M. Yeast (2001) [Pubmed]
  7. Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Yoshida, S., Asakawa, K., Toh-e, A. Curr. Biol. (2002) [Pubmed]
  8. Nuclear pore complex antigens delineate nuclear envelope dynamics in vegetative and conjugating Saccharomyces cerevisiae. Copeland, C.S., Snyder, M. Yeast (1993) [Pubmed]
  9. Crm1-mediated nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast. Bembenek, J., Kang, J., Kurischko, C., Li, B., Raab, J.R., Belanger, K.D., Luca, F.C., Yu, H. Cell Cycle (2005) [Pubmed]
  10. The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules. Cho, H.P., Liu, Y., Gomez, M., Dunlap, J., Tyers, M., Wang, Y. Mol. Cell. Biol. (2005) [Pubmed]
  11. Dbf2 is implicated in a Cbt1-dependent pathway following a shift from glucose to galactose or non-fermentable carbon sources in Saccharomyces cerevisiae. Grandin, N., Charbonneau, M. Mol. Gen. Genet. (1999) [Pubmed]
  12. Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14. Shirayama, M., Matsui, Y., Toh-e, A. Mol. Gen. Genet. (1996) [Pubmed]
  13. The activity of Cdc14p, an oligomeric dual specificity protein phosphatase from Saccharomyces cerevisiae, is required for cell cycle progression. Taylor, G.S., Liu, Y., Baskerville, C., Charbonneau, H. J. Biol. Chem. (1997) [Pubmed]
  14. CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle. Wan, J., Xu, H., Grunstein, M. J. Biol. Chem. (1992) [Pubmed]
  15. Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. Pereira, G., Manson, C., Grindlay, J., Schiebel, E. J. Cell Biol. (2002) [Pubmed]
  16. Characterization of the Net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. Traverso, E.E., Baskerville, C., Liu, Y., Shou, W., James, P., Deshaies, R.J., Charbonneau, H. J. Biol. Chem. (2001) [Pubmed]
  17. A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis. Geil, C., Schwab, M., Seufert, W. Curr. Biol. (2008) [Pubmed]
  18. Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Visintin, R., Hwang, E.S., Amon, A. Nature (1999) [Pubmed]
  19. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Stoepel, J., Ottey, M.A., Kurischko, C., Hieter, P., Luca, F.C. Mol. Biol. Cell (2005) [Pubmed]
  20. Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Pereira, G., Schiebel, E. Science (2003) [Pubmed]
  21. A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae. Asakawa, K., Toh-e, A. Genetics (2002) [Pubmed]
  22. Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W., Jang, J., Shevchenko, A., Charbonneau, H., Deshaies, R.J. Cell (1999) [Pubmed]
  23. Phosphorylation and spindle pole body localization of the Cdc15p mitotic regulatory protein kinase in budding yeast. Xu, S., Huang, H.K., Kaiser, P., Latterich, M., Hunter, T. Curr. Biol. (2000) [Pubmed]
  24. The Saccharomyces cerevisiae Cdc14 phosphatase is implicated in the structural organization of the nucleolus. de Almeida, A., Raccurt, I., Peyrol, S., Charbonneau, M. Biol. Cell (1999) [Pubmed]
  25. Assaying the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Yellman, C.M., Burke, D.J. Methods Mol. Biol. (2004) [Pubmed]
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