High impact information on PRP6

• PRP6 and PRP9 wild-type alleles have been sequenced [1].
• In addition, PRP6 and PRP9 exhibit leucine repeat motifs which may be implicated in protein interactions [1].
• Both approaches indicate that PRP6 encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein and that the PRP9 protein is required for a stable U2 snRNP-substrate interaction [2].
• The suppressor was selected for its ability to alleviate simultaneously the temperature-sensitive growth phenotypes of rna2 and rna6 [3].
• The amino acid sequence of Prp1p shares 29.6% identity and 50.6% similarity with that of the PRP6 protein of Saccharomyces cerevisiae, which is a component of the U4/U6 snRNP required for spliceosome assembly [4].

Biological context of PRP6

• Both PRP6 and PRP9 proteins have cysteine/histidine motifs loosely related to those found in zinc finger proteins [1].
• From a yeast genomic library, we have isolated plasmids that complement prp6 or prp9 ts mutations [5].
• Constitutive splicing of the potato invertase mini-exon 2 (9 nt long) requires a branchpoint sequence positioned around 50 nt upstream of the 5' splice site of the adjacent intron and a U(11) element found just downstream of the branchpoint in the upstream intron [Simpson, Hedley, Watters, Clark, McQuade, Machray and Brown (2000) RNA 6, 422-433] [6].

Associations of PRP6 with chemical compounds

• The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors [1].

Other interactions of PRP6

• Furthermore, in preliminary experiments rna3 and our four new alleles of it, together with rna6 and dbf5 and 6, showed enhanced spontaneous mutation frequency [7].

References