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Gene Review

PRP5  -  DEAD-box RNA helicase PRP5

Saccharomyces cerevisiae S288c

Synonyms: Pre-mRNA-processing ATP-dependent RNA helicase PRP5, RNA5, YBR1603, YBR237W
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Disease relevance of PRP5


High impact information on PRP5

  • In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction [2].
  • Loss of CUS2 does not bypass the role of PRP5, an RNA helicase family member required for ATP-dependent prespliceosome formation [3].
  • The requirement for ATP and the helicase-like PRP5 protein suggests that these factors might promote a conformational change (involving either the U1 or U2 snRNP) that is required for the association of U2 snRNP with the pre-mRNA [4].
  • A 96-kDa protein predicted by the DNA sequence of the Saccharomyces cerevisiae PRP5 gene contains a domain that bears a striking resemblance to a family of RNA helicases characterized by the conserved amino acid sequence Asp-Glu-Ala-Asp (D-E-A-D) [5].
  • Previous work indicated that the product of the PRP5 gene is required for splicing and that spliceosome assembly does not occur in its absence [5].

Biological context of PRP5

  • To examine the role of PRP5 in splicing, we cloned the gene by complementation of a temperature-sensitive mutation and determined its DNA sequence [5].
  • Moreover, the COOH-terminal region of 78 residues of HEL117 is 38.5% identical and 59% similar to the COOH-terminal region of a yeast PRP5 protein that is involved in RNA splicing [6].

Associations of PRP5 with chemical compounds


Physical interactions of PRP5

  • We infer that the presence of Cus2p and stem IIa-destabilized forms of U2 small nuclear RNA places high demands on the ATP-driven function of Prp5p [8].

Other interactions of PRP5

  • We constructed yeast strains with pairwise combinations of 28 different U2 alleles with 10 prp mutations and found lethal double-mutant combinations with prp5, -9, -11, and -21 but not with prp3, -4, -8, or -19 [9].
  • A directed two-hybrid screen reveals a network of prospective interactions that includes previously unreported intra-SF3b contacts and SF3b interactions with the RES subunit Bud13p, the Prp5p DExD/H-box protein, Mud2p, and the late-acting nineteen complex [10].
  • An RNaseH assay in extracts was used to demonstrate that Prp5 mediates an ATP-dependent conformational change in the intact U2 small nuclear ribonucleoprotein [11].
  • Debranching this RNA in vitro with Dbr1p creates an uncapped version of the normal Ty1 RNA 5' end [12].
  • The transfer RNA 5' maturation enzyme RNase P has been characterized in Bacteria, Archaea, and Eukarya [13].


  1. Neurospora crassa nuclear genome contains analogy of Saccharomyces cerevisiae genes for ribosomal RNA processing. Dutta, S.K., Verma, M., Verma, M. Curr. Genet. (1987) [Pubmed]
  2. Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56. Zhang, M., Green, M.R. Genes Dev. (2001) [Pubmed]
  3. ATP can be dispensable for prespliceosome formation in yeast. Perriman, R., Ares, M. Genes Dev. (2000) [Pubmed]
  4. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Ruby, S.W., Chang, T.H., Abelson, J. Genes Dev. (1993) [Pubmed]
  5. PRP5: a helicase-like protein required for mRNA splicing in yeast. Dalbadie-McFarland, G., Abelson, J. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  6. A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor. Sukegawa, J., Blobel, G. J. Biol. Chem. (1995) [Pubmed]
  7. Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor. Schwelnus, W., Richert, K., Opitz, F., Gross, T., Habara, Y., Tani, T., Käufer, N.F. EMBO Rep. (2001) [Pubmed]
  8. ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA. Perriman, R., Barta, I., Voeltz, G.K., Abelson, J., Ares, M. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  9. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Wells, S.E., Ares, M. Mol. Cell. Biol. (1994) [Pubmed]
  10. Interactions of the yeast SF3b splicing factor. Wang, Q., He, J., Lynn, B., Rymond, B.C. Mol. Cell. Biol. (2005) [Pubmed]
  11. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. O'Day, C.L., Dalbadie-McFarland, G., Abelson, J. J. Biol. Chem. (1996) [Pubmed]
  12. RNA branching and debranching in the yeast retrovirus-like element Ty1. Cheng, Z., Menees, T.M. Science (2004) [Pubmed]
  13. Chloroplast ribonuclease P does not utilize the ribozyme-type pre-tRNA cleavage mechanism. Thomas, B.C., Li, X., Gegenheimer, P. RNA (2000) [Pubmed]
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