High impact information on SOH1

• Bioinformatic analysis traces the Soh1/MED31 family of Mediator subunits to the point of major eukaryotic divergence, before the appearance of the canonical heptapeptide repeat structure of the RNA polymerase II C-terminal domain [1].
• Because mutations in SOH1, RPB2 and SUA7 suppress the hyperrecombination phenotype of hpr1 mutants, this suggests a link between recombination in direct repeats and transcription [2].
• Interestingly, SOH1 not only interacts with factors involved in DNA repair, but transcription as well [2].
• The soh1, soh2 and soh4 mutants were isolated as suppressors of the temperature-dependent growth of the hyperrecombination mutant hpr1 of Saccharomyces cerevisiae [2].
• The Soh1 protein interacts with a DNA repair protein, Rad5p, in a two-hybrid system assay [2].

Associations of SOH1 with chemical compounds

• We here demonstrated that the Soh1/MED31 protein is a stable component of Mediator complex isolated from Schizosaccharomyces pombe and Saccharomyces cerevisiae [1].

Analytical, diagnostic and therapeutic context of SOH1

• The two-hybrid analysis, together with co-immunoprecipitation studies and gel filtration experiments revealed that Med31 (Soh1) is associated with the yeast Mediator that therefore comprises 25 subunits [3].

References