The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

RPB2  -  DNA-directed RNA polymerase II core...

Saccharomyces cerevisiae S288c

Synonyms: B150, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit RPB2, RNA polymerase II subunit 2, RPB150, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of RPB2


High impact information on RPB2

  • Although multiple functions have been assigned to the three largest subunits, RPB1, RPB2, and RPB3, the functions of the remaining smaller subunits are unknown [3].
  • The RPB2 phylogeny shows with significant statistical support that taxa in Pezizomycotina with ascohymenial ontogeny (ascoma generally forms after nuclear pairing) are ancestral and paraphyletic, whereas ascolocular fungi with fissitunicate asci are a clade derived from them [4].
  • Using high-resolution repair analysis, we determined the in vivo repair kinetics of cyclobutane pyrimidine dimers positioned around the transcription initiation site of RNAPII-transcribed genes RPB2 and URA3 [5].
  • We found that repair of cyclobutane pyrimidine dimers in the transcribed strand of the expressed RPB2 gene in the chromosome of a repair-proficient strain is much more rapid than that in the nontranscribed strand [6].
  • This gene, RPB2, exists in a single copy in the haploid genome [1].

Chemical compound and disease context of RPB2

  • Supported by analogous results obtained for E. coli RNA polymerase using derivative VIII (2), we hypothesized that the second domain labeled by this derivative in the B150 subunit was domain I. Mutagenesis of the unique lysine present in domain I demonstrated that Lys 1102 was the target of derivative VIII [7].

Biological context of RPB2

  • The gene for this subunit is present in single copy and maps to chromosome XV, where two other yeast RNA polymerase II subunits, RPB2 and RPB8, reside [8].
  • Transcription-coupled and global genome repair in the Saccharomyces cerevisiae RPB2 gene at nucleotide resolution [9].
  • A plasmid shuffle method was used to screen thoroughly for mutations in RPB2 that suppress a temperature-sensitive mutation, rpb1-1, which is located in region H of RPB1 [10].
  • We investigated whether suppressor genetics could provide evidence for interactions between specific segments of RPB1 and RPB2 in Saccharomyces cerevisiae [10].
  • We have cloned and sequenced the cDNA encoding the open reading frame of the mRNA of the second largest subunit of RNA polymerase II, or RPB2, of tomato [11].

Associations of RPB2 with chemical compounds

  • Glycerol gradient fractionation of extracts of cells pulse-labeled for various times revealed that a subcomplex of RPB2 and RPB3 appears soon after subunit synthesis and can be chased into fully assembled enzyme [12].
  • Sequence analysis of the rpb2-100 suppressor defined a cysteine replacement of the phylogenetically invariant arginine residue at position 512 (R512C), located within homology block D of Rpb2 [13].
  • The repair of UV-induced photoproducts (cyclobutane pyrimidine dimers) in a well-characterized minichromosome, genomic DNA, and a transcribed genomic gene (RPB2) of a rad23delta mutant of Saccharomyces care was examined [14].
  • Predicted zinc-binding motifs have been noted in the NH2-terminal part of B220 and the COOH-terminal region of B150 subunits [15].

Other interactions of RPB2

  • When the results obtained with the RPB2 gene can be generalized, the RAD7 and RAD16 proteins not only are essential for repair of silenced regions but also function in repair of nontranscribed strands of active genes in S. cerevisiae [16].
  • The soh1, soh2 and soh4 mutants were isolated as suppressors of the temperature-dependent growth of the hyperrecombination mutant hpr1 of Saccharomyces cerevisiae [17].
  • SOH2 is identical to RPB2, which encodes the second largest subunit of RNA polymerase II, and SOH4 is the same as SUA7, encoding the yeast transcription initiation factor TFIIB [17].
  • In cross-linking reactions dependent on transcription, RPB1, RPB2, and RPB5 were cross-linked with N(3)RdUTP [18].
  • Additionally, the rate of repair of the transcribed and nontranscribed strands of the yeast RPB2 gene in the rad28 mutant is identical to that observed in wild-type cells following exposure to UV light [19].

Analytical, diagnostic and therapeutic context of RPB2

  • Immunoprecipitation from 32P-labeled cell extracts revealed that three of the subunits, RPB1, RPB2, and RPB6, are phosphorylated in vivo [20].
  • A 5586bp sequence (accession no. ), which includes the RNA polymerase II gene (RPB2) encoding the second largest protein subunit (RPB2), was obtained from the wheat biotype Phaeosphaeria nodorum (PN-w) by PCR amplification [21].
  • Site-directed mutagenesis in the predicted metal-binding domain of B150 demonstrated its role in zinc binding [15].


  1. Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Sweetser, D., Nonet, M., Young, R.A. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  2. Defects in yeast RNA polymerase II transcription elicit hypersensitivity to G1 arrest induced by Kluyveromyces lactis zymocin. Kitamoto, H.K., Jablonowski, D., Nagase, J., Schaffrath, R. Mol. Genet. Genomics (2002) [Pubmed]
  3. RNA polymerase II subunit RPB9 is required for accurate start site selection. Hull, M.W., McKune, K., Woychik, N.A. Genes Dev. (1995) [Pubmed]
  4. Body plan evolution of ascomycetes, as inferred from an RNA polymerase II phylogeny. Liu, Y.J., Hall, B.D. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  5. Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae. Tijsterman, M., Verhage, R.A., van de Putte, P., Tasseron-de Jong, J.G., Brouwer, J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  6. Preferential repair of cyclobutane pyrimidine dimers in the transcribed strand of a gene in yeast chromosomes and plasmids is dependent on transcription. Sweder, K.S., Hanawalt, P.C. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  7. Determination of lysine residues affinity labeled in the active site of yeast RNA polymerase II(B) by mutagenesis. Treich, I., Carles, C., Sentenac, A., Riva, M. Nucleic Acids Res. (1992) [Pubmed]
  8. RNA polymerase II subunit RPB10 is essential for yeast cell viability. Woychik, N.A., Young, R.A. J. Biol. Chem. (1990) [Pubmed]
  9. Transcription-coupled and global genome repair in the Saccharomyces cerevisiae RPB2 gene at nucleotide resolution. Tijsterman, M., Tasseron-de Jong, J.G., van de Putte, P., Brouwer, J. Nucleic Acids Res. (1996) [Pubmed]
  10. Genetic exploration of interactive domains in RNA polymerase II subunits. Martin, C., Okamura, S., Young, R. Mol. Cell. Biol. (1990) [Pubmed]
  11. Sequence analysis of the second largest subunit of tomato RNA polymerase II. Warrilow, D., Symons, R.H. Plant Mol. Biol. (1996) [Pubmed]
  12. Mutations in the three largest subunits of yeast RNA polymerase II that affect enzyme assembly. Kolodziej, P.A., Young, R.A. Mol. Cell. Biol. (1991) [Pubmed]
  13. Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae. Pappas, D.L., Hampsey, M. Mol. Cell. Biol. (2000) [Pubmed]
  14. Rad23 is required for transcription-coupled repair and efficient overrall repair in Saccharomyces cerevisiae. Mueller, J.P., Smerdon, M.J. Mol. Cell. Biol. (1996) [Pubmed]
  15. Zinc-binding subunits of yeast RNA polymerases. Treich, I., Riva, M., Sentenac, A. J. Biol. Chem. (1991) [Pubmed]
  16. The RAD7 and RAD16 genes, which are essential for pyrimidine dimer removal from the silent mating type loci, are also required for repair of the nontranscribed strand of an active gene in Saccharomyces cerevisiae. Verhage, R., Zeeman, A.M., de Groot, N., Gleig, F., Bang, D.D., van de Putte, P., Brouwer, J. Mol. Cell. Biol. (1994) [Pubmed]
  17. Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae. Fan, H.Y., Cheng, K.K., Klein, H.L. Genetics (1996) [Pubmed]
  18. Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking. Wooddell, C.I., Burgess, R.R. Biochemistry (2000) [Pubmed]
  19. Molecular cloning and characterization of Saccharomyces cerevisiae RAD28, the yeast homolog of the human Cockayne syndrome A (CSA) gene. Bhatia, P.K., Verhage, R.A., Brouwer, J., Friedberg, E.C. J. Bacteriol. (1996) [Pubmed]
  20. RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Kolodziej, P.A., Woychik, N., Liao, S.M., Young, R.A. Mol. Cell. Biol. (1990) [Pubmed]
  21. RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria. Malkus, A., Linda Chang, P.F., Zuzga, S.M., Chung, K.R., Shao, J., Cunfer, B.M., Arseniuk, E., Ueng, P.P. Mycol. Res. (2006) [Pubmed]
WikiGenes - Universities