High impact information on OKP1

• COMA, MIND, and the previously described Ndc80 complex constitute three independent and essential platforms onto which outer kinetochore proteins assemble [1].
• Applying this screen we have identified three proteins-Ctf19, Mcm21, and the product of an unspecified open reading frame that we named Okp1-as components of the budding yeast centromere [2].
• A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1 [3].
• Only mutations that affect the CBF3 cycle, and not mutants in outer kinetochore subunits, cause defects in septins [4].
• Implication of a novel multiprotein Dam1p complex in outer kinetochore function [5].

Biological context of OKP1

• Based on our results, we propose that Ame1 and Okp1 are required for a sustained checkpoint arrest in the presence of mis-segregated chromosomes [6].
• These physical interaction dependencies provide insights into the molecular architecture and centromere DNA loading requirements of the outer kinetochore complex [7].
• In budding yeast, a subset of kinetochore proteins, referred to as the outer kinetochore, provides a link between centromere DNA-binding proteins of the inner kinetochore and microtubule-binding proteins [7].

Anatomical context of OKP1

• These and other analyses implicate the four newly identified subunits and the Dam1p complex as a whole in outer kinetochore function where they are well positioned to facilitate the association of chromosomes with spindle microtubules [5].

Other interactions of OKP1

• Overexpression of VPS9, BTS1 and OKP1 suppressed the carboxypeptidase Y secretion defect of deltabph1 [8].
• Spindle checkpoint maintenance requires Ame1 and Okp1 [6].
• Chl4p and iml3p are two new members of the budding yeast outer kinetochore [7].
• The Dam1 complex, also known as DASH complex, is the outer kinetochore protein complex of yeast that plays a crucial role in attachment of kinetochore to microtubule [9].

References