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Gene Review

CEP3  -  Cep3p

Saccharomyces cerevisiae S288c

Synonyms: CBF3, CBF3B, CSL1, Centromere DNA-binding protein complex CBF3 subunit B, Centromere protein 3, ...
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High impact information on CEP3

  • We report the reconstitution of Cbf3p from recombinant proteins and an analysis of its p58Ctf13 and p23Skp1 subunits. p23Skp1 has both G1- and G2-specific functions in yeast and binds to p58Ctf13 and to the essential Cdc4p component of the ubiquitin conjugating complex Scul(Cdc4) [1].
  • In S. cerevisiae, the four-protein Cbf3 complex binds to the essential CDEIII region of centromeric DNA to initiate kinetochore assembly [1].
  • We propose that coupled activation and destruction link the assembly of Cbf3p to the duplication of centromeres in S phase [1].
  • We show that the function of p23Skp1 in Cbf3p is to activate p58Ctf13 by phosphorylation. p58Ctf13 is an unstable protein that is targeted to the proteosome, probably by Scul(Cdc4)-mediated ubiquitination [1].
  • The budding yeast SKP1 gene, identified as a dosage suppressor of a known kinetochore protein mutant, encodes an intrinsic 22.3 kDa subunit of CBF3, a multiprotein complex that binds centromere DNA in vitro [2].

Biological context of CEP3


Anatomical context of CEP3


Associations of CEP3 with chemical compounds

  • Deletion of PLC1, as well as plc1 mutations which abrogate the interaction of Plc1p with the CBF3 complex, results in a higher frequency of minichromosome loss, nocodazole sensitivity, and mitotic delay [4].
  • The budding yeast PLC1 gene encodes a homolog of the delta isoform of mammalian phosphoinositide-specific phospholipase C. Here, we present evidence that Plc1p associates with the kinetochore complex CBF3 [4].
  • We find that CBF3 is absolutely essential for this attachment but, contrary to previous reports (Hyman, A. A., K. Middleton, M. Centola, T.J. Mitchison, and J. Carbon. 1992. Microtubule-motor activity of a yeast centromere-binding protein complex. Nature (Lond.). 359:533-536) is not sufficient [5].

Physical interactions of CEP3

  • Specifically, the Ndc10p component of the centromere DNA-binding CBF3 complex is altered by the glc7-10 mutation; Ndc10p is hyperphosphorylated in glc7-10 extracts [7].
  • Consistent with this possibility, we find that CBF3 interacts with Bir1p, the homologue of the passenger protein Survivin [8].

Regulatory relationships of CEP3

  • These results demonstrate a novel role for CBF3 in regulating cytokinesis, a role that is reminiscent of passenger proteins [8].
  • Specifically, we show that septin ring separation and disassembly is delayed in anaphase, suggesting that CBF3 regulates septin dynamics [8].

Other interactions of CEP3


Analytical, diagnostic and therapeutic context of CEP3


  1. Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phosphorylation. Kaplan, K.B., Hyman, A.A., Sorger, P.K. Cell (1997) [Pubmed]
  2. Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression. Connelly, C., Hieter, P. Cell (1996) [Pubmed]
  3. CEP3 encodes a centromere protein of Saccharomyces cerevisiae. Strunnikov, A.V., Kingsbury, J., Koshland, D. J. Cell Biol. (1995) [Pubmed]
  4. Phospholipase C is involved in kinetochore function in Saccharomyces cerevisiae. Lin, H., Choi, J.H., Hasek, J., DeLillo, N., Lou, W., Vancura, A. Mol. Cell. Biol. (2000) [Pubmed]
  5. Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro. Sorger, P.K., Severin, F.F., Hyman, A.A. J. Cell Biol. (1994) [Pubmed]
  6. Microtubule-motor activity of a yeast centromere-binding protein complex. Hyman, A.A., Middleton, K., Centola, M., Mitchison, T.J., Carbon, J. Nature (1992) [Pubmed]
  7. Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. Sassoon, I., Severin, F.F., Andrews, P.D., Taba, M.R., Kaplan, K.B., Ashford, A.J., Stark, M.J., Sorger, P.K., Hyman, A.A. Genes Dev. (1999) [Pubmed]
  8. A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis. Gillis, A.N., Thomas, S., Hansen, S.D., Kaplan, K.B. J. Cell Biol. (2005) [Pubmed]
  9. SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Kitagawa, K., Skowyra, D., Elledge, S.J., Harper, J.W., Hieter, P. Mol. Cell (1999) [Pubmed]
  10. KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Middleton, K., Carbon, J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  11. The unstable F-box protein p58-Ctf13 forms the structural core of the CBF3 kinetochore complex. Russell, I.D., Grancell, A.S., Sorger, P.K. J. Cell Biol. (1999) [Pubmed]
  12. Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Pietrasanta, L.I., Thrower, D., Hsieh, W., Rao, S., Stemmann, O., Lechner, J., Carbon, J., Hansma, H. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
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