Disease relevance of HYS2

• When POL31 and POL32 were co-expressed in Escherichia coli, a tetrameric (Pol31p.Pol32p)2 species was detected by gel filtration, indicating that the two subunits form a complex [1].

High impact information on HYS2

• DNA polymerase delta (Pol delta) from Saccharomyces cerevisiae consists of three subunits, Pol3 (125 kDa), Pol31 (55 kDa), and Pol32 (40 kDa), present at a 1:1:1 stoichiometry in purified preparations [2].
• In this paper we determine partial amino acid sequences of the 125 and 55 kDa polypeptides and find that they match parts of the amino acid sequences predicted from the nucleotide sequence of the CDC2 and HYS2 genes respectively [3].
• HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae [4].
• Taken together, these data suggest that Hys2 plays a role in DNA replication [4].
• Surprisingly, deletions of RAD18 and MGS1 aggravated the temperature sensitivity conferred by most ts or cs alleles and specifically suppressed the hys2-1 and hys2-1-like mutations of POL31 [5].

Biological context of HYS2

• Saccharomyces cerevisiae DNA polymerase delta (Poldelta) is a heterotrimeric enzyme consisting of Pol3 (the catalytic subunit), Pol31 and Pol32 [5].

Physical interactions of HYS2

• We also show by Western blotting that Hys2 protein co-purifies with DNA polymerase III activity as well as Cdc2 polypeptide [3].

Other interactions of HYS2

• Moreover, regions I and VII of Pol31, which are specifically sensitive to high levels of Mgs1 and PCNA, could be sites of interaction [5].

References