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Gene Review

POL32  -  Pol32p

Saccharomyces cerevisiae S288c

Synonyms: DNA polymerase delta subunit 3, J1626, YJR043C
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Disease relevance of POL32


High impact information on POL32

  • The pif1delta dna2delta strain remains methylmethane sulfonate sensitive and temperature sensitive; however, these phenotypes can be suppressed by further deletion of a subunit of pol delta, POL32 [2].
  • The formation of MNNG-induced mutations is almost abolished in the rad30Delta pol32Delta double mutant of yeast, which lacks the RAD30 gene that encodes Poleta and the Pol32 subunit of DNA polymerase delta (Poldelta) [3].
  • The amino-terminal region of Pol32 is essential for damage-induced mutagenesis [4].
  • DNA polymerase delta (Pol delta) from Saccharomyces cerevisiae consists of three subunits, Pol3 (125 kDa), Pol31 (55 kDa), and Pol32 (40 kDa), present at a 1:1:1 stoichiometry in purified preparations [5].
  • Previously, based on gel filtration studies of Pol delta, we suggested that the enzyme may be a dimer of catalytic cores, with dimerization mediated by the Pol32 subunit (Burgers, P. M., and Gerik, K. J. (1998) J. Biol. Chem. 273, 19756-19762) [5].

Biological context of POL32


Associations of POL32 with chemical compounds

  • The POL32 gene is not essential, but a deletion mutant shows cold sensitivity for growth and is sensitive to hydroxyurea and DNA damaging agents [1].
  • The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer [10].

Physical interactions of POL32


Other interactions of POL32

  • We also found that the increase in the CAN1 spontaneous forward mutation of different rad mutators relies entirely or partially on a functional POL32 gene [6].
  • In addition, lethality was observed when the POL32 deletion mutation was combined with conditional mutations in either the POL3 or POL31 gene [1].
  • Simultaneous deletion of POL32 and SRS2 dramatically decreases cellular viability at 15 degrees C and greatly increases cellular sensitivity to hydroxyurea at the permissive temperature [6].
  • Based on these findings, we propose that POL32 defines a link between the DNA polymerase and helicase activities, and plays a role in the mutagenic bypass repair pathway [6].
  • Here, by measuring the CAN1 forward mutation rate, we found that either POL32 or REV3 (which encodes the Pol zeta catalytic subunit) inactivation produces overlapping antimutator effects against rad mutators belonging to three epistasis groups [8].


  1. Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta. Gerik, K.J., Li, X., Pautz, A., Burgers, P.M. J. Biol. Chem. (1998) [Pubmed]
  2. Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta. Budd, M.E., Reis, C.C., Smith, S., Myung, K., Campbell, J.L. Mol. Cell. Biol. (2006) [Pubmed]
  3. Replication past O(6)-methylguanine by yeast and human DNA polymerase eta. Haracska, L., Prakash, S., Prakash, L. Mol. Cell. Biol. (2000) [Pubmed]
  4. The Pol32 subunit of DNA polymerase delta contains separable domains for processive replication and proliferating cell nuclear antigen (PCNA) binding. Johansson, E., Garg, P., Burgers, P.M. J. Biol. Chem. (2004) [Pubmed]
  5. Structure of DNA polymerase delta from Saccharomyces cerevisiae. Johansson, E., Majka, J., Burgers, P.M. J. Biol. Chem. (2001) [Pubmed]
  6. POL32, a subunit of the Saccharomyces cerevisiae DNA polymerase delta, defines a link between DNA replication and the mutagenic bypass repair pathway. Huang, M.E., de Calignon, A., Nicolas, A., Galibert, F. Curr. Genet. (2000) [Pubmed]
  7. Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31. Vijeh Motlagh, N.D., Seki, M., Branzei, D., Enomoto, T. DNA Repair (Amst.) (2006) [Pubmed]
  8. Pol32, a subunit of Saccharomyces cerevisiae DNA polymerase delta, suppresses genomic deletions and is involved in the mutagenic bypass pathway. Huang, M.E., Rio, A.G., Galibert, M.D., Galibert, F. Genetics (2002) [Pubmed]
  9. The Saccharomyces cerevisiae protein YJR043C (Pol32) interacts with the catalytic subunit of DNA polymerase alpha and is required for cell cycle progression in G2/M. Huang, M.E., Le Douarin, B., Henry, C., Galibert, F. Mol. Gen. Genet. (1999) [Pubmed]
  10. The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer. Gibbs, P.E., McDonald, J., Woodgate, R., Lawrence, C.W. Genetics (2005) [Pubmed]
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