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Gene Review

MGS1  -  ssDNA-dependent ATPase MGS1

Saccharomyces cerevisiae S288c

Synonyms: DNA-dependent ATPase MGS1, Maintenance of genome stability protein 1, N1302, YNL218W
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Disease relevance of MGS1

  • Here we report a Saccharomyces cerevisiae gene, MGS1 (Maintenance of Genome Stability 1), which encodes a protein belonging to the AAA(+) class of ATPases, and whose central region is similar to Escherichia coli RuvB, a Holliday junction branch migration motor protein [1].

High impact information on MGS1

  • Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway [2].
  • Saccharomyces cerevisiae Mgs1 protein, which possesses DNA-dependent ATPase and single strand DNA annealing activities, plays a role in maintaining genomic stability [2].
  • The mgs1 mutant is not sensitive to DNA-damaging agents, but the mgs1 rad5 double mutant has increased sensitivity to hydroxyurea and a greatly increased spontaneous mutation rate [2].
  • The mgs1 mutation is synergistic with a mutation in top3 (encoding topoisomerase III), and the double mutant exhibits severe growth defects and markedly increased genome instability [1].
  • Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance [3].

Biological context of MGS1

  • The data presented raise the possibility that the absence of MGS1 may impair the processing of Okazaki fragments, leading to genomic instability [4].
  • Three other interacting proteins, Mgs1p, Ulp2, and Plp2, participate in chromosome assembling and segregation, whereas the function of two others has not been established [5].
  • Characterization of the slow-growth phenotype of S. cerevisiae Whip/Mgs1 Sgs1 double deletion mutants [6].
  • Our results suggest that Whip/Mgs1 is implicated in DNA metabolism, and is required for normal growth and cell cycle progression in the absence of Sgs1 [6].
  • N1302 contains the consensus sequence for a phosphate-binding loop common to ATP- and GTP-binding proteins and a strictly conserved 'SRC' sequence of unknown function present in all accessory proteins of replicative polymerases [7].

Physical interactions of MGS1

  • We also demonstrate that Mgs1 interacts physically with Pol31 in vivo [8].

Regulatory relationships of MGS1

  • Surprisingly, deletions of RAD18 and MGS1 aggravated the temperature sensitivity conferred by most ts or cs alleles and specifically suppressed the hys2-1 and hys2-1-like mutations of POL31 [8].
  • Mgs1 stimulates the structure-specific nuclease activity of Rad27 (yeast Fen1 or yFen1) in an ATP-dependent manner [4].

Other interactions of MGS1

  • Replication fork bypass processes appear to be highly conserved within eukaryotes, with homologs for SGS1 and MGS1 found in both Schizosaccharomyces pombe and mammalian cells [9].
  • Moreover, regions I and VII of Pol31, which are specifically sensitive to high levels of Mgs1 and PCNA, could be sites of interaction [8].
  • In this study, we identified MGS1 as a multicopy suppressor of the temperature-sensitive dna2Delta405N mutation, a DNA2 allele lacking the N-terminal 405 amino acid residues [4].
  • We found that the RFC-like motifs in Whip/Mgs1 are essential for its function [10].


  1. A yeast gene, MGS1, encoding a DNA-dependent AAA(+) ATPase is required to maintain genome stability. Hishida, T., Iwasaki, H., Ohno, T., Morishita, T., Shinagawa, H. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  2. Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway. Hishida, T., Ohno, T., Iwasaki, H., Shinagawa, H. EMBO J. (2002) [Pubmed]
  3. Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Hishida, T., Ohya, T., Kubota, Y., Kamada, Y., Shinagawa, H. Mol. Cell. Biol. (2006) [Pubmed]
  4. In vivo and in vitro studies of Mgs1 suggest a link between genome instability and Okazaki fragment processing. Kim, J.H., Kang, Y.H., Kang, H.J., Kim, D.H., Ryu, G.H., Kang, M.J., Seo, Y.S. Nucleic Acids Res. (2005) [Pubmed]
  5. Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex. Wysocka, M., Rytka, J., Kurlandzka, A. Exp. Cell Res. (2004) [Pubmed]
  6. Characterization of the slow-growth phenotype of S. cerevisiae Whip/Mgs1 Sgs1 double deletion mutants. Branzei, D., Seki, M., Onoda, F., Yagi, H., Kawabe, Y., Enomoto, T. DNA Repair (Amst.) (2002) [Pubmed]
  7. The sequence of a 13.5 kb DNA segment from the left arm of yeast chromosome XIV reveals MER1; RAP1; a new putative member of the DNA replication complex and a new putative serine/threonine phosphatase gene. Coster, F., Van Dyck, L., Jonniaux, J.L., Purnelle, B., Goffeau, A. Yeast (1995) [Pubmed]
  8. Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31. Vijeh Motlagh, N.D., Seki, M., Branzei, D., Enomoto, T. DNA Repair (Amst.) (2006) [Pubmed]
  9. Regulation of alternative replication bypass pathways at stalled replication forks and its effects on genome stability: a yeast model. Barbour, L., Xiao, W. Mutat. Res. (2003) [Pubmed]
  10. The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase delta. Branzei, D., Seki, M., Onoda, F., Enomoto, T. Mol. Genet. Genomics (2002) [Pubmed]
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