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Gene Review

UBA1  -  E1 ubiquitin-activating protein UBA1

Saccharomyces cerevisiae S288c

Synonyms: Ubiquitin-activating enzyme E1 1, YKL210W
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High impact information on UBA1

  • We report the isolation and functional analysis of the gene (UBA1) for the ubiquitin-activating enzyme of the yeast Saccharomyces cerevisiae [1].
  • UBA1 encodes a 114 kd protein whose amino acid sequence contains motifs characteristic of nucleotide-binding sites [1].
  • Deletion of the UBA1 gene is lethal, demonstrating that the formation of ubiquitin--protein conjugates is essential for cell viability [1].
  • We describe the isolation and analysis of another essential gene, termed UBA2, that encodes a 71-kDa protein with extensive sequence similarities to both the UBA1-encoded yeast E1 and E1 enzymes of other organisms [2].
  • In the yeast Saccharomyces cerevisiae, a 114-kDa E1 enzyme is encoded by an essential gene termed UBA1 (McGrath, J.P., Jentsch, S., and Varshavsky, A. (1991) EMBO J. 10, 227-236) [2].

Biological context of UBA1


Physical interactions of UBA1

  • Interestingly, the surfaces of UbL and Ub that bind to UBA1, UBA2, and PUbS2 are similar, consisting of five beta-strands and their connecting loops [3].

Other interactions of UBA1

  • Surprisingly, these genes encode proteins with antagonistic activity as two, UBA1 and UBA2, are ubiquitin-activating enzymes whereas the other three are de-ubiquitinating hydrolases [4].
  • Whereas the amino acid sequence of UBA2 is nearly identical to UBA1, the sequence of UBA3 is significantly different [5].

Analytical, diagnostic and therapeutic context of UBA1


  1. UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme. McGrath, J.P., Jentsch, S., Varshavsky, A. EMBO J. (1991) [Pubmed]
  2. An essential yeast gene encoding a homolog of ubiquitin-activating enzyme. Dohmen, R.J., Stappen, R., McGrath, J.P., Forrová, H., Kolarov, J., Goffeau, A., Varshavsky, A. J. Biol. Chem. (1995) [Pubmed]
  3. Binding surface mapping of intra- and interdomain interactions among hHR23B, ubiquitin, and polyubiquitin binding site 2 of S5a. Ryu, K.S., Lee, K.J., Bae, S.H., Kim, B.K., Kim, K.A., Choi, B.S. J. Biol. Chem. (2003) [Pubmed]
  4. Isolation and characterization of KIUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae. Winkler, A.A., Korstanje, R., Zonneveld, B.J., Hooykaas, P.J., Steensma, H.Y. Curr. Genet. (2000) [Pubmed]
  5. Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis. Hatfield, P.M., Vierstra, R.D. J. Biol. Chem. (1992) [Pubmed]
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