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Gene Review

PRP40  -  Prp40p

Saccharomyces cerevisiae S288c

Synonyms: Pre-mRNA-processing protein PRP40, YKL012W, YKL165
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High impact information on PRP40

  • In yeast, this bridging involves interactions between the WW domains in the splicing factor PRP40 and a proline-rich domain in the branchpoint binding protein, BBP [1].
  • Therefore, we conclude that PRP40 encodes a novel, essential splicing component that associates with the yeast U1 small nuclear ribonucleoprotein particle [2].
  • A gene disruption experiment shows that PRP40 is an essential gene [2].
  • The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains [3].
  • Furthermore, we found that both Prp40 WW domains interact with PPxY motifs (where x is any residue) present in peptides derived from the splicing factors BBP and Prp8 [4].

Biological context of PRP40


Physical interactions of PRP40

  • In addition, U1 RNA coimmunoprecipitates with Pro40, indicating that Prp40 is bound to the U1 small nuclear ribonucleoprotein particle in vivo [2].

Analytical, diagnostic and therapeutic context of PRP40

  • Sequence alignments and phylogenetic tree reconstructions using the FF domains of three functionally related proteins, Prp40, FBP11, and CA150, revealed that Prp40 and FBP11 are not orthologous proteins and supported the different ligand specificities shown by their respective FF1 domains [3].
  • Domain dissection studies reveal that phospho-CTD binding occurs at multiple locations in Prp40, including sites in both the WW and FF domain regions [7].


  1. WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. Bedford, M.T., Reed, R., Leder, P. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  2. Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particle. Kao, H.Y., Siliciano, P.G. Mol. Cell. Biol. (1996) [Pubmed]
  3. The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains. Gasch, A., Wiesner, S., Martin-Malpartida, P., Ramirez-Espain, X., Ruiz, L., Macias, M.J. J. Biol. Chem. (2006) [Pubmed]
  4. Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40. Wiesner, S., Stier, G., Sattler, M., Macias, M.J. J. Mol. Biol. (2002) [Pubmed]
  5. The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing. Murphy, M.W., Olson, B.L., Siliciano, P.G. Genetics (2004) [Pubmed]
  6. The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10. Pascolo, S., Ghazvini, M., Boyer, J., Colleaux, L., Thierry, A., Dujon, B. Yeast (1992) [Pubmed]
  7. The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II. Morris, D.P., Greenleaf, A.L. J. Biol. Chem. (2000) [Pubmed]
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