High impact information on ITR2

• By the complementation of a yeast mutant defective in myo-inositol transport (Nikawa, J., Nagumo, T., and Yamashita, S. (1982) J. Bacteriol. 150, 441-446), we isolated two myo-inositol transporter genes, ITR1 and ITR2, from a yeast gene library [1].
• The ITR1 product was the major transporter and the ITR2 product the minor one in cells grown in minimum medium containing glucose [1].
• The results show that the expression of the two ITR genes is differently regulated: ITR1 was repressed by inositol and choline whereas ITR2 was constitutive [2].
• The predicted Git1p has similarity to a variety of S. cerevisiae transporters, including a phosphate transporter (Pho84p), and both inositol transporters (Itr1p and Itr2p) [3].
• The itr2 gene is essential for cell growth and its mRNA level is repressed by glucose [4].

Biological context of ITR2

• The ITR1 and ITR2 genes contained long open reading frames capable of encoding 584 and 612 amino acids with calculated relative molecular masses of 63,605 and 67,041, respectively [1].

Anatomical context of ITR2

• Fluorescence microscopy analysis indicated that both GFP-fused Itr1p and Itr2p are localized to the plasma membrane [5].

Other interactions of ITR2

• Itr1p and Itr2p are high and low affinity transporters, respectively [5].

Analytical, diagnostic and therapeutic context of ITR2

• Northern blot analysis showed that ITR1 mRNA was much more abundant than ITR2 mRNA [1].

References