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Gene Review

ITR1  -  myo-inositol transporter ITR1

Saccharomyces cerevisiae S288c

Synonyms: D9719.3, Myo-inositol transporter 1, YDR497C
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High impact information on ITR1

  • The interactions between two myosin proteins, UNC-54 and MYO-1, and ITR-1 were identified in a yeast two-hybrid screen and subsequently confirmed in vivo and in vitro [1].
  • The decrease was due to repression of ITR1 transcription, independent of the negative regulator Opi1p, and degradation of the existing permease [2].
  • Uptake of inositol by Saccharomyces cerevisiae is mediated by a specific inositol permease encoded by the ITR1 gene [3].
  • Thus, inactivation of the Itr1 permease is accompanied by endocytic internalization followed by degradation in the vacuole [3].
  • By the complementation of a yeast mutant defective in myo-inositol transport (Nikawa, J., Nagumo, T., and Yamashita, S. (1982) J. Bacteriol. 150, 441-446), we isolated two myo-inositol transporter genes, ITR1 and ITR2, from a yeast gene library [4].

Biological context of ITR1

  • A multi-copy plasmid that expresses GFP-fused Itr1p under the control of the original ITR1 promoter was constructed [5].
  • The ITR1 and ITR2 genes contained long open reading frames capable of encoding 584 and 612 amino acids with calculated relative molecular masses of 63,605 and 67,041, respectively [4].
  • The HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 5'-TAACAAC-3' or 5'-CAACTAAC-3' sequences, derived from the promoter regions of the endosperm-specific genes Hor2 and Itr1, encoding a B-hordein and trypsin-inhibitor BTI-CMe, respectively [6].

Anatomical context of ITR1


Associations of ITR1 with chemical compounds


Regulatory relationships of ITR1

  • The results show that the expression of the two ITR genes is differently regulated: ITR1 was repressed by inositol and choline whereas ITR2 was constitutive [9].

Other interactions of ITR1

  • Gene-disruption experiments revealed that DIE1 was essential for the expression of ITR1 but that DIE2 was not [7].
  • Itr1p and Itr2p are high and low affinity transporters, respectively [5].
  • In this work, a yeast strain has been constructed in which HIS3 expression is controlled by the ITR1 promoter [7].
  • Chimeric genes that express Itr1p and Itr2p fused to the green fluorescent protein (GFP) under the control of the ADH1 promoter were constructed [5].

Analytical, diagnostic and therapeutic context of ITR1


  1. A direct interaction between IP(3) receptors and myosin II regulates IP(3) signaling in C. elegans. Walker, D.S., Ly, S., Lockwood, K.C., Baylis, H.A. Curr. Biol. (2002) [Pubmed]
  2. Inositol transport in Saccharomyces cerevisiae is regulated by transcriptional and degradative endocytic mechanisms during the growth cycle that are distinct from inositol-induced regulation. Robinson, K.S., Lai, K., Cannon, T.A., McGraw, P. Mol. Biol. Cell (1996) [Pubmed]
  3. Regulation of inositol transport in Saccharomyces cerevisiae involves inositol-induced changes in permease stability and endocytic degradation in the vacuole. Lai, K., Bolognese, C.P., Swift, S., McGraw, P. J. Biol. Chem. (1995) [Pubmed]
  4. Isolation and characterization of two distinct myo-inositol transporter genes of Saccharomyces cerevisiae. Nikawa, J., Tsukagoshi, Y., Yamashita, S. J. Biol. Chem. (1991) [Pubmed]
  5. Mutational analysis and localization of the inositol transporters of Saccharomyces cerevisiae. Miyashita, M., Shugyo, M., Nikawa, J. J. Biosci. Bioeng. (2003) [Pubmed]
  6. The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development. Diaz, I., Vicente-Carbajosa, J., Abraham, Z., Martínez, M., Isabel-La Moneda, I., Carbonero, P. Plant J. (2002) [Pubmed]
  7. Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae. Nikawa, J., Hosaka, K. Mol. Microbiol. (1995) [Pubmed]
  8. Dual control of inositol transport in Saccharomyces cerevisiae by irreversible inactivation of permease and regulation of permease synthesis by INO2, INO4, and OPI1. Lai, K., McGraw, P. J. Biol. Chem. (1994) [Pubmed]
  9. Differential regulation of two myo-inositol transporter genes of Saccharomyces cerevisiae. Nikawa, J., Hosaka, K., Yamashita, S. Mol. Microbiol. (1993) [Pubmed]
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