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Gene Review

PHO84  -  Pho84p

Saccharomyces cerevisiae S288c

Synonyms: Inorganic phosphate transporter PHO84, YM7056.03C, YML123C
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Disease relevance of PHO84


High impact information on PHO84

  • This differential kinetic behavior is largely eliminated in cells that lack the ability to store phosphate internally in the form of polyphosphate, but the threshold of external phosphate required for induction of PHO5 and PHO84 is unaffected [3].
  • In the budding yeast Saccharomyces cerevisiae, PHO84 and PHO86 are among the genes that are most highly induced in response to phosphate starvation [4].
  • Here, we show that the subcellular localization of Pho84p is regulated in response to extracellular phosphate levels; it is localized to the plasma membrane in low-phosphate medium but quickly endocytosed and transported to the vacuole upon addition of phosphate to the medium [4].
  • Upon expression, both AtPT1 and AtPT2 were able to complement the pho84 mutant phenotype of yeast strain NS219 lacking the high-affinity phosphate transport activity [5].
  • For this study, using a formaldehyde-based in vivo cross-linking and chromatin immunoprecipitation (ChIP) assay, we have analyzed the role of Ubp8p in the regulation of H3-K4 methylation at three other SAGA-dependent yeast genes, namely, PHO84, ADH1, and CUP1 [6].

Biological context of PHO84


Anatomical context of PHO84


Associations of PHO84 with chemical compounds

  • In a search for other genes involved in manganese homeostasis, PHO84 was identified [1].
  • In this study, we have compared the phosphate-responsive mechanism of cells expressing PHO84 with a Deltapho84 strain by use of a phosphate analogue, methylphosphonate, which was judged to be suitable for assessment of phosphate homeostasis in the cells [11].
  • These results demonstrate that phosphate acts as a nutrient signal for activation of the protein kinase A pathway in yeast in a glucose-dependent way and they indicate that the Pho84 and Pho87 carriers act as specific phosphate sensors for rapid phosphate signalling [12].
  • Intriguingly, in this study we found a tight correlation between selenite resistance and expression of the high affinity orthophosphate carrier Pho84p [13].
  • The predicted Git1p has similarity to a variety of S. cerevisiae transporters, including a phosphate transporter (Pho84p), and both inositol transporters (Itr1p and Itr2p) [14].

Regulatory relationships of PHO84

  • The constitutive rAPase+ phenotype of the pho86 pho87 mutant was partially suppressed by an increased dosage of the PHO84 gene [9].

Other interactions of PHO84

  • The high-affinity system consists of Pho84p and Pho89p [15].
  • The latter phenotypes were shown to be due to a defect in Pi uptake, and the Gtr1 protein was found to be functionally associated with the Pho84 Pi transporter [7].
  • In Saccharomyces cerevisiae, the phosphate signal transduction pathway (PHO pathway) is known to regulate the expression of several phosphate-responsive genes, such as PHO5 and PHO84 [16].
  • Deletion of MOT2 also caused increased transcription of unrelated genes such as GAL7 and PHO84 [17].
  • Under these conditions, cells lacking both Pho84p and the high affinity Smf1p transporter accumulated low levels of manganese, although there was no major effect on activity of manganese-requiring enzymes [1].


  1. The Saccharomyces cerevisiae high affinity phosphate transporter encoded by PHO84 also functions in manganese homeostasis. Jensen, L.T., Ajua-Alemanji, M., Culotta, V.C. J. Biol. Chem. (2003) [Pubmed]
  2. Studies of cytochrome c oxidase-driven H(+)-coupled phosphate transport catalyzed by the Saccharomyces cerevisiae Pho84 permease in coreconstituted vesicles. Fristedt, U., van Der Rest, M., Poolman, B., Konings, W.N., Persson, B.L. Biochemistry (1999) [Pubmed]
  3. An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels. Thomas, M.R., O'Shea, E.K. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  4. Pho86p, an endoplasmic reticulum (ER) resident protein in Saccharomyces cerevisiae, is required for ER exit of the high-affinity phosphate transporter Pho84p. Lau, W.T., Howson, R.W., Malkus, P., Schekman, R., O'Shea, E.K. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  5. Phosphate transporters from the higher plant Arabidopsis thaliana. Muchhal, U.S., Pardo, J.M., Raghothama, K.G. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  6. Ubp8p, a histone deubiquitinase whose association with SAGA is mediated by Sgf11p, differentially regulates lysine 4 methylation of histone H3 in vivo. Shukla, A., Stanojevic, N., Duan, Z., Sen, P., Bhaumik, S.R. Mol. Cell. Biol. (2006) [Pubmed]
  7. Putative GTP-binding protein, Gtr1, associated with the function of the Pho84 inorganic phosphate transporter in Saccharomyces cerevisiae. Bun-Ya, M., Harashima, S., Oshima, Y. Mol. Cell. Biol. (1992) [Pubmed]
  8. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Bun-Ya, M., Nishimura, M., Harashima, S., Oshima, Y. Mol. Cell. Biol. (1991) [Pubmed]
  9. Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Bun-ya, M., Shikata, K., Nakade, S., Yompakdee, C., Harashima, S., Oshima, Y. Curr. Genet. (1996) [Pubmed]
  10. A putative new membrane protein, Pho86p, in the inorganic phosphate uptake system of Saccharomyces cerevisiae. Yompakdee, C., Bun-ya, M., Shikata, K., Ogawa, N., Harashima, S., Oshima, Y. Gene (1996) [Pubmed]
  11. Effects of methylphosphonate, a phosphate analogue, on the expression and degradation of the high-affinity phosphate transporter Pho84, in Saccharomyces cerevisiae. Pratt, J.R., Mouillon, J.M., Lagerstedt, J.O., Pattison-Granberg, J., Lundh, K.I., Persson, B.L. Biochemistry (2004) [Pubmed]
  12. Inorganic phosphate is sensed by specific phosphate carriers and acts in concert with glucose as a nutrient signal for activation of the protein kinase A pathway in the yeast Saccharomyces cerevisiae. Giots, F., Donaton, M.C., Thevelein, J.M. Mol. Microbiol. (2003) [Pubmed]
  13. Low affinity orthophosphate carriers regulate PHO gene expression independently of internal orthophosphate concentration in Saccharomyces cerevisiae. Pinson, B., Merle, M., Franconi, J.M., Daignan-Fornier, B. J. Biol. Chem. (2004) [Pubmed]
  14. GIT1, a gene encoding a novel transporter for glycerophosphoinositol in Saccharomyces cerevisiae. Patton-Vogt, J.L., Henry, S.A. Genetics (1998) [Pubmed]
  15. Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae. Auesukaree, C., Homma, T., Kaneko, Y., Harashima, S. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  16. Intracellular phosphate serves as a signal for the regulation of the PHO pathway in Saccharomyces cerevisiae. Auesukaree, C., Homma, T., Tochio, H., Shirakawa, M., Kaneko, Y., Harashima, S. J. Biol. Chem. (2004) [Pubmed]
  17. The yeast MOT2 gene encodes a putative zinc finger protein that serves as a global negative regulator affecting expression of several categories of genes, including mating-pheromone-responsive genes. Irie, K., Yamaguchi, K., Kawase, K., Matsumoto, K. Mol. Cell. Biol. (1994) [Pubmed]
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