High impact information on MND2

• In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes [1].
• The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1 [1].
• Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase [2].
• Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis [1].
• We show that Mnd2, a factor known to associate with APC components, prevents premature securin degradation in meiosis by APC(Ama1). mnd2Delta cells lack linear chromosome axes and exhibit precocious sister chromatid separation, but deletion of AMA1 suppresses these defects [2].

Associations of MND2 with chemical compounds

• Similar to the MND2 deletion strain, cells harboring the alanine mutant that did not form spores arrested after premeiotic S phase with a single undivided nucleus and low levels of the APC(Ama1) meiotic substrate, Clb5, relative to wild type cells [3].

Biological context of MND2

• Both Mnd2 and Swm1 were found specifically associated with a highly purified preparation of APC from haploid yeast whole cell extract [4].

Other interactions of MND2

• Previous studies described meiotic defects for mutations in MND2 and SWM1 [4].
• During S- and prophase, Mnd2 prevents activation of the APC/C by a meiosis-specific activator called Ama1 [1].

References