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Gene Review

PDS1  -  Pds1p

Saccharomyces cerevisiae S288c

Synonyms: Securin, YD9727.08C, YDR113C
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High impact information on PDS1


Biological context of PDS1

  • This metaphase block was partially relieved by a deletion of PDS1 [5].
  • Previous studies indicate that RAD9 is required for X-ray-associated sister chromatid exchange (SCE), suppresses homology-directed translocations, and is involved in pathways for double-strand break repair (DSB) repair that are different than those controlled by PDS1 [6].
  • We show that destruction of Pds1p is the APC's sole role in triggering Scc1p's dissociation from chromatids and that Pds1p forms a stable complex with a 180 kDa protein called Esp1p, which is essential for the dissociation of Scc1p from sister chromatids and for their separation [4].
  • Inappropriate attachment/tension between chromosomal kinetochores and the kinetochore microtubules activates the spindle assembly checkpoint, which delays anaphase by blocking the ubiquitin-mediated degradation of securin/Pds1p by APC(Cdc20) [7].
  • In contrast, Rad53p and Chk1p, needed for the stabilization of Pds1p in the context of the G2 DNA-damage checkpoint pathway, are dispensable [8].

Anatomical context of PDS1


Associations of PDS1 with chemical compounds


Physical interactions of PDS1

  • Pds1p was also stabilized by the overexpression of Ibd2p in wild-type cells [15].
  • Here we find that Pds1 interacts directly with Cdc20 and that this interaction requires Pds1's destruction box [16].

Enzymatic interactions of PDS1


Regulatory relationships of PDS1


Other interactions of PDS1

  • We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p [4].
  • How the parallel pathways might interact ultimately to achieve arrest is not yet understood, but we do provide evidence that neither the RAD53 nor the PDS1 pathway appears to maintain arrest by inhibiting adaptation [22].
  • RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast [22].
  • While the G(1)/S block in both cdc4-12 and cdc4Delta mutants is abolished by the deletion of the SIC1 gene (causing the mutants to be arrested predominantly in G(2)/M), the preanaphase arrest in the cdc4-12 mutant is relieved by the deletion of PDS1 [23].
  • Therefore, Rad23p and Ddi1p participate in a subset of Pds1p-dependent cell cycle controls [24].

Analytical, diagnostic and therapeutic context of PDS1


  1. How do so few control so many? Nasmyth, K. Cell (2005) [Pubmed]
  2. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Oelschlaegel, T., Schwickart, M., Matos, J., Bogdanova, A., Camasses, A., Havlis, J., Shevchenko, A., Zachariae, W. Cell (2005) [Pubmed]
  3. Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast. Alexandru, G., Uhlmann, F., Mechtler, K., Poupart, M.A., Nasmyth, K. Cell (2001) [Pubmed]
  4. An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast. Ciosk, R., Zachariae, W., Michaelis, C., Shevchenko, A., Mann, M., Nasmyth, K. Cell (1998) [Pubmed]
  5. Smt3/SUMO and Ubc9 are required for efficient APC/C-mediated proteolysis in budding yeast. Dieckhoff, P., Bolte, M., Sancak, Y., Braus, G.H., Irniger, S. Mol. Microbiol. (2004) [Pubmed]
  6. Saccharomyces cerevisiae RAD53 (CHK2) but not CHK1 is required for double-strand break-initiated SCE and DNA damage-associated SCE after exposure to X rays and chemical agents. Fasullo, M., Dong, Z., Sun, M., Zeng, L. DNA Repair (Amst.) (2005) [Pubmed]
  7. Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint. Burton, J.L., Solomon, M.J. Genes Dev. (2007) [Pubmed]
  8. Mec1p regulates Pds1p levels in S phase: complex coordination of DNA replication and mitosis. Clarke, D.J., Segal, M., Jensen, S., Reed, S.I. Nat. Cell Biol. (2001) [Pubmed]
  9. Macromolecular assemblies regulate nonvesicular phosphatidylserine traffic in yeast. Choi, J.Y., Riekhof, W.R., Wu, W.I., Voelker, D.R. Biochem. Soc. Trans. (2006) [Pubmed]
  10. The Saccharomyces cerevisiae PDS1 and RAD9 checkpoint genes control different DNA double-strand break repair pathways. DeMase, D., Zeng, L., Cera, C., Fasullo, M. DNA Repair (Amst.) (2005) [Pubmed]
  11. Securin is not required for cellular viability, but is required for normal growth of mouse embryonic fibroblasts. Mei, J., Huang, X., Zhang, P. Curr. Biol. (2001) [Pubmed]
  12. The Pds1 anaphase inhibitor and Mec1 kinase define distinct checkpoints coupling S phase with mitosis in budding yeast. Clarke, D.J., Segal, M., Mondésert, G., Reed, S.I. Curr. Biol. (1999) [Pubmed]
  13. Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast. Salah, S.M., Nasmyth, K. Chromosoma (2000) [Pubmed]
  14. S-phase cyclins are required for a stable arrest at metaphase. Meyn, M.A., Holloway, S.L. Curr. Biol. (2000) [Pubmed]
  15. IBD2 encodes a novel component of the Bub2p-dependent spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Hwang, H.S., Song, K. Genetics (2002) [Pubmed]
  16. The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner. Hilioti, Z., Chung, Y.S., Mochizuki, Y., Hardy, C.F., Cohen-Fix, O. Curr. Biol. (2001) [Pubmed]
  17. Pds1 phosphorylation in response to DNA damage is essential for its DNA damage checkpoint function. Wang, H., Liu, D., Wang, Y., Qin, J., Elledge, S.J. Genes Dev. (2001) [Pubmed]
  18. A role for Saccharomyces cerevisiae Chk1p in the response to replication blocks. Schollaert, K.L., Poisson, J.M., Searle, J.S., Schwanekamp, J.A., Tomlinson, C.R., Sanchez, Y. Mol. Biol. Cell (2004) [Pubmed]
  19. Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae. Karumbati, A.S., Wilson, T.E. Genetics (2005) [Pubmed]
  20. The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. Shirayama, M., Zachariae, W., Ciosk, R., Nasmyth, K. EMBO J. (1998) [Pubmed]
  21. Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase. Schott, E.J., Hoyt, M.A. Genetics (1998) [Pubmed]
  22. RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. Gardner, R., Putnam, C.W., Weinert, T. EMBO J. (1999) [Pubmed]
  23. Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Goh, P.Y., Surana, U. Mol. Cell. Biol. (1999) [Pubmed]
  24. Dosage suppressors of pds1 implicate ubiquitin-associated domains in checkpoint control. Clarke, D.J., Mondesert, G., Segal, M., Bertolaet, B.L., Jensen, S., Wolff, M., Henze, M., Reed, S.I. Mol. Cell. Biol. (2001) [Pubmed]
  25. Assaying the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Yellman, C.M., Burke, D.J. Methods Mol. Biol. (2004) [Pubmed]
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