High impact information on REC114

• We have also found that the Mei4 and Rec114 proteins localize to foci on chromosomes and these foci partially colocalize with each other and with Mer2 [1].
• Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation [1].
• Some of the observed phenotypes of the disruption mutant and the N-terminal sequence homology suggest that Rec7p is a functional homolog of Rec114p of Saccharomyces cerevisiae [2].
• The low viability of spores produced by dmc1 mutants carrying high copy numbers of REC114 is rescued when reductional segregation is bypassed by mutation of spo13 [3].
• The splicing of REC114 does not require MER1, a gene known to be involved in meiosis-specific RNA processing [4].

Biological context of REC114

• This work demonstrates that REC114 does have an intron and is one of only three genes in yeast with introns located in the 3' end [4].
• One of these genes, REC114, is described here, and the data confirm that REC114 is a meiosis-specific recombination gene with no detectable function in mitosis [5].

Other interactions of REC114

• We propose that Mer2, Mei4, and Rec114 form a distinct complex required for DSB formation [1].
• REC114 is located on chromosome XIII approximately 4.9 cM from CIN4 [5].

References