High impact information on SPO13

• Establishment of this Sgo1-binding domain requires the 120-base-pair (bp) core centromere, the kinetochore component Bub1, and the meiosis-specific factor Spo13 [1].
• Overexpression of SPO13 inhibits anaphase onset by at least two mechanisms [2].
• We have determined the molecular basis for the metaphase arrest obtained when SPO13 is overexpressed during the mitotic cell cycle [2].
• First, Spo13 causes a transient delay in degradation of the anaphase inhibitor Pds1 [2].
• The budding yeast protein Spo13 plays a key role in preventing centromeric cohesin from being lost during meiosis I [2].

Biological context of SPO13

• Ume3p mutants resistant to degradation resulted in a 2-fold reduction in SPO13 mRNA levels during meiosis, indicating that the down-regulation of this cyclin is important for normal meiotic gene expression [3].
• The ume3-1 allele was identified as a mutation that allowed the aberrant expression of several meiotic genes (e.g. SPO11, SPO13) during mitotic cell division in Saccharomyces cerevisiae [3].
• HO endonuclease was expressed under the control of the meiotic-specific SPO13 promoter, creating a DSB at a single site on one of yeast's 16 chromosomes [4].
• The spores produced by SPO13 diploids homozygous for hop1 were largely inviable, as expected for a defect in interhomolog recombination that results in high levels of nondisjunction [5].
• Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae [6].

Associations of SPO13 with chemical compounds

• The meiotic block is not bypassed in backgrounds homozygous for spo13, rad50 delta, or rad9 delta mutations, but is bypassed in the presence of hydroxyurea, a drug known to inhibit DNA chain elongation [7].

Regulatory relationships of SPO13

• SPO13 negatively regulates the progression of mitotic and meiotic nuclear division in Saccharomyces cerevisiae [8].
• The mre4 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass the reductional division [9].

Other interactions of SPO13

• The msc (meiotic sister-chromatid recombination) mutants were quantified in spo13 meiosis with respect to meiotic unequal SCR frequency, disome segregation pattern, sporulation frequency, and spore viability [10].
• Although substantial meiotic recombination was found for these conditional alleles at the restrictive temperature, the level of exchange measured in spo13 meiosis was reduced in some of the monitored intervals, indicating that nondisjunction resulting from a deficit in crossing over could account for SPO13 spore inviability [11].
• A mutation at the REC102 locus was identified in a screen for yeast mutants that produce inviable spores. rec102 spore lethality is rescued by a spo13 mutation, which causes cells to bypass the meiosis I division [12].
• The spore inviability of rad58 strains is not rescued by a spo13 mutation [13].
• Diploids homozygous for a mek1 null mutation produce only 13% viable spores. mek1 spore inviability is rescued by a spo13 mutation, which causes cells to bypass the meiosis I division [14].

References