High impact information on DBF20

• Thus, Dbf2/Dbf20 kinase activity is precisely regulated in the cell cycle by a post-translational mechanism and phosphorylates its target substrates for an event that occurs during anaphase and/or telophase [1].
• Relocalization of these proteins from spindle pole bodies to mother daughter neck seems to be necessary for this role because cdc15-2 mutant cells, though capable of exiting mitosis at semipermissive temperature, are unable to localize Dbf2 (and Dbf20) to the "neck" and fail to undergo cytokinesis [2].
• Here we present genetic evidence that nonfunctional mutant Dbf2 protein blocks the function of Dbf20 protein by sequestering a common interacting protein encoded by SPO12 [3].

Biological context of DBF20

• In contrast to the DBF2 mRNA, which is expressed under cell-cycle control at or near START [Johnston et al., Mol. Cell. Biol. 10 (1990) 1358-1366], the DBF20 mRNA is expressed at a low level and not under cell-cycle control [4].
• The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition [1].

Associations of DBF20 with chemical compounds

• The kinase activity is specific for serine/threonine residues and Dbf2 accounts for the bulk of the activity, with Dbf20 playing a minor role [1].

Other interactions of DBF20

• The DBF2 and DBF20 genes of the budding yeast Saccharomyces cerevisiae encode a pair of structurally similar protein kinases [3].

References