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ORC6  -  origin recognition complex subunit 6

Saccharomyces cerevisiae S288c

Synonyms: AAP1, ACS-associated protein 1, Origin recognition complex 50 kDa subunit, Origin recognition complex subunit 6, YHR118C
 
 
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High impact information on ORC6

  • Matches between its predicted amino acid sequence and peptide sequence obtained from the 50-kilodalton subunit of the yeast origin recognition complex (ORC) established that the gene isolated here, ORC6, encodes this subunit [1].
  • Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch [2].
  • An essential role for Orc6 in DNA replication was identified by depleting it at specific cell cycle stages [3].
  • An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes [3].
  • Only the Orc1-5 subunits appear to be required for origin binding in budding yeast, yet Orc6 is an essential protein for cell proliferation [3].
 

Biological context of ORC6

  • ORC6 protein level did not change through the cell cycle [4].
  • Several cellular proteins co-immunoprecipitated with ORC6, including a 65-kDa protein that was hyperphosphorylated in G(1) and dephosphorylated in mitosis [4].
  • The human ORC6 gene is located on chromosome 16q12 [4].
  • The Clb5-Orc6 interaction requires replication initiation, and is maintained throughout the remainder of S phase and into M phase [2].
 

Physical interactions of ORC6

  • We demonstrate that two regions of Orc6 bind Cdt1 directly, and that the extreme C terminus of Orc6 (Orc6-CTD) interacts tightly with the remaining five ORC subunits [5].
 

Other interactions of ORC6

  • Trace amounts of nonphosphorylatable Cdc6 are dominant lethal in strains bearing nonphosphorylatable Orc2 and Orc6, apparently because of rereplication [6].
  • Eliminating the Clb5-Orc6 interaction has no effect on initiation of replication but instead sensitizes cells to lethal overreplication [2].
  • Orc6 was not detected at the site of division between mother and daughter cells, in contrast to observations for metazoans, and is not required for mitosis or cytokinesis [3].

References

  1. Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Li, J.J., Herskowitz, I. Science (1993) [Pubmed]
  2. Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch. Wilmes, G.M., Archambault, V., Austin, R.J., Jacobson, M.D., Bell, S.P., Cross, F.R. Genes Dev. (2004) [Pubmed]
  3. An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes. Semple, J.W., Da-Silva, L.F., Jervis, E.J., Ah-Kee, J., Al-Attar, H., Kummer, L., Heikkila, J.J., Pasero, P., Duncker, B.P. EMBO J. (2006) [Pubmed]
  4. Identification and characterization of the human ORC6 homolog. Dhar, S.K., Dutta, A. J. Biol. Chem. (2000) [Pubmed]
  5. Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading. Chen, S., de Vries, M.A., Bell, S.P. Genes Dev. (2007) [Pubmed]
  6. Roles of the CDK Phosphorylation Sites of Yeast Cdc6 in Chromatin Binding and Rereplication. Honey, S., Futcher, B. Mol. Biol. Cell (2007) [Pubmed]
 
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