Disease relevance of hmw1

• A spontaneous hemadsorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1 [1].

High impact information on hmw1

• However, the hmw1 and hmw3 genes were transcribed and translated at wild-type levels in hmw2 mutants [2].
• Stability of Mycoplasma pneumoniae cytadherence-accessory protein HMW1 correlates with its association with the triton shell [3].
• The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of unknown function [4].
• In contrast, mutant M6 transformed with a recombinant transposon expressing the wild-type hmw1 allele exhibited a near-normal morphology and localized P1 to the attachment organelle [5].

Biological context of hmw1

• In addition, HMW1 membrane topography was examined for antibody accessibility on the mycoplasmal surface. hmw3 and hmw1 flank four open reading frames (ORFs) spanning approximately 4.3 kb and in the same orientation as the hmw genes [6].

Analytical, diagnostic and therapeutic context of hmw1

• Southern blot hybridizations confirmed insertion of the transposon and the presence of both the resident and recombinant hmw1 alleles [7].
• Analysis by Western immunoblotting revealed a truncated HMW1 (HMW1') in the transformants, the level of HMW1' being dependent upon the orientation of the hmw1 gene in the transposon and the site of insertion [7].
• M. pneumoniae protein profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) following culturing in the presence of [3H]Pro were consistent with the high Pro content predicted for HMW3 and indicated the same for cytadherence accessory protein HMW1 [8].

References