Disease relevance of argF

• Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene [1].
• E. coli carrying a P. aeruginosa argF+ recombinant plasmid produced mRNA-1 with low efficiency but no (or very little) mRNA-2 [1].
• The pts mutations in four independently isolated mutant strains exhibited from 11 to 20% linkage to argF, and one of these mutations exhibited 3% linkage to lys-9015 in phage F116L-mediated transductional crosses [2].

High impact information on argF

• Inactivation of argR abolished arginine control of the biosynthetic enzymes encoded by the car and argF operons [3].
• Gel retardation experiments yielded apparent dissociation constants of 2.5 x 10(-11), 4.2 x 10(-12), and 7.2 x 10(-11) M for carA, argF, and aruC operators, respectively [4].
• Purification and characterization of an arginine regulatory protein, ArgR, from Pseudomonas aeruginosa and its interactions with the control regions for the car, argF, and aru operons [4].
• Genetic mapping experiments revealed that algG was closely linked (greater than 90%) to argF, which lies at 34 min on the P. aeruginosa chromosome and is adjacent to a cluster of genes required for alginate biosynthesis [5].
• At the nucleotide sequence level, the P. aeruginosa argF gene had 52.4% identity with the arcB gene [1].

Chemical compound and disease context of argF

• In the argF promoter region no sequence homologous to the "arg box" (arginine operator module) of E. coli was found [1].

Biological context of argF

• We have determined the complete nucleotide sequence of the argF gene including its promoter-control region [1].
• DNase I footprinting showed that ArgR protects a region of 45 to 47 bp that overlaps the promoters for the biosynthetic car and argF operons, indicating that ArgR exerts its negative control on the expression of these operons by steric hindrance [4].
• Mutations at a locus designated pts, which resulted in a loss of PTS activity, exhibited 57% linkage to argF at 55 min on the chromosome in plasmid R68.45-mediated conjugational crosses [2].

Associations of argF with chemical compounds

• Arginine repressed argF transcription in P. aeruginosa [1].

Regulatory relationships of argF

• The corresponding arcB mutant alleles on a multicopy plasmid specifically suppressed an argF mutation of P. aeruginosa [6].

References