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Gene Review

ECs4367  -  hypothetical protein

Escherichia coli O157:H7 str. Sakai

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Disease relevance of ECs4367

  • A gene encoding the universal stress protein (uspA) was cloned from Escherichia coli O157:H7 isolated from a Jack-in-a-Box outbreak [1].
  • Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence [2].

High impact information on ECs4367

  • The synthesis of the small, cytoplasmic protein UspA universal stress protein A) of Escherichia coli is induced as soon as the cell growth rate falls below the maximal growth rate supported by the medium, regardless of the condition inhibiting growth [3].
  • The increase in the level of UspA during growth arrest was found to be primarily a result of transcriptional activation of the corresponding gene [4].
  • To determine further the mode of regulation of the protein designated UspA (universal stress protein A) we cloned the gene encoding the protein by the technique of reverse genetics [4].
  • In vivo, three isoforms of UspA were detected, two of which were phosphorylated as determined by alkaline phosphatase treatment; in vitro, phosphorylation with [gamma-32P]ATP yielded two radioactive UspA isoforms [5].
  • A hypothesis invoking RpoS and UspA (universal stress protein, also significantly elevated in minimal glucose medium) as playing a role in coordinating these various aspects and consequences of glucose and acetate metabolism was generated [6].

Biological context of ECs4367

  • Within an 800-base pair (bp) region sequenced, a 15-bp mismatch existed between E. coli K-12 and O157:H7, of which 11 base pairs were internal to the structural gene of uspA [1].
  • It was found that some CDPs induced during the growth-arrested phase belong to the heat-shock, oxidation stress, SOS and stringent response regulons, while others are general stress inducible proteins (e.g. H-NS, UspA) [7].
  • The pleiotrophic phenotype revealed for this mutant and the similarity of the C-terminal region of ORF280 to UspA from E. coli indicates that the A. brasilense ORF280 may be a Usp-like protein [8].
  • Furthermore, UspA makes an important contribution to the in vivo virulence of Salmonella in mice thus highlighting the importance of stress resistance regulation in pathogenicity and survival within the host [2].
  • An in silico screen of the Salmonella genome sequence revealed that Salmonella typhimurium LT2 possesses a homologue belonging to the universal stress protein A (UspA) family [2].

Analytical, diagnostic and therapeutic context of ECs4367

  • In addition, electrophoretic analysis together with V8 protease peptide mapping demonstrated that the pIs of some specific proteins became more acidic as a result of the elevation of the levels of UspA [9].
  • To formulate a mucosal vaccine against these pathogens, recombinant lipidated P4 (rLP4) and P6 (rLP6) proteins of NTHi and ubiquitous cell surface protein A (UspA) of M. catarrhalis were used for active immunization experiments in a mouse nasal challenge model [10].


  1. Cloning and sequencing of the gene encoding universal stress protein from Escherichia coli O157:H7 isolated from Jack-in-a-Box outbreak. Chen, J., Griffiths, M.W. Lett. Appl. Microbiol. (1999) [Pubmed]
  2. Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence. Liu, W.T., Karavolos, M.H., Bulmer, D.M., Allaoui, A., Hormaeche, R.D., Lee, J.J., Anjam Khan, C.M. Microb. Pathog. (2007) [Pubmed]
  3. Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest. Nyström, T., Neidhardt, F.C. Mol. Microbiol. (1994) [Pubmed]
  4. Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Nyström, T., Neidhardt, F.C. Mol. Microbiol. (1992) [Pubmed]
  5. The universal stress protein, UspA, of Escherichia coli is phosphorylated in response to stasis. Freestone, P., Nyström, T., Trinei, M., Norris, V. J. Mol. Biol. (1997) [Pubmed]
  6. Functional genomics: expression analysis of Escherichia coli growing on minimal and rich media. Tao, H., Bausch, C., Richmond, C., Blattner, F.R., Conway, T. J. Bacteriol. (1999) [Pubmed]
  7. The cadmium-stress stimulon of Escherichia coli K-12. Ferianc, P., Farewell, A., Nyström, T. Microbiology (Reading, Engl.) (1998) [Pubmed]
  8. An Azospirillum brasilense Tn5 mutant with modified stress response and impaired in flocculation. Galindo Blaha, C.A., Schrank, I.S. Antonie Van Leeuwenhoek (2003) [Pubmed]
  9. Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12. Nyström, T., Neidhardt, F.C. J. Bacteriol. (1996) [Pubmed]
  10. Reduction of nasal colonization of nontypeable Haemophilus influenzae following intranasal immunization with rLP4/rLP6/UspA2 proteins combined with aqueous formulation of RC529. Mason, K.W., Zhu, D., Scheuer, C.A., McMichael, J.C., Zlotnick, G.W., Green, B.A. Vaccine (2004) [Pubmed]
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