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Gene Review

modA  -  molybdate ABC transporter periplasmic...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0752, JW0746
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Disease relevance of modA


High impact information on modA

  • Among the various oxyanions tested, only tungstate replaced molybdate in the repression of modA by ModE, but the affinity of ModE-tungstate for modABCD operator DNA was 6 times lower than with ModE-molybdate [2].
  • Mutants in modA or modBC were unable to grow using nitrate or Mo-nitrogenase [3].
  • However, relative to modA, binding of ModE to the moaA promoter appeared to be largely molybdate independent both in vitro and in vivo [4].
  • Compared to the full-length protein, the N-terminal half of ModE has a greatly diminished capacity to bind the modA promoter in vitro and to repress expression from a modA-lacZ operon fusion in vivo [5].
  • This level was increased by over 175 times by a mutation in the modA, modB, or modC gene [6].

Biological context of modA


Associations of modA with chemical compounds


Analytical, diagnostic and therapeutic context of modA

  • Utilizing an in vivo titration assay, we localized the ModE-binding site to the start of modA transcription [4].


  1. The molybdate-binding protein (ModA) of the plant pathogen Xanthomonas axonopodis pv. citri. Balan, A., Santacruz, C.P., Moutran, A., Ferreira, R.C., Medrano, F.J., P??rez, C.A., Ramos, C.H., Ferreira, L.C. Protein Expr. Purif. (2006) [Pubmed]
  2. An analysis of the binding of repressor protein ModE to modABCD (molybdate transport) operator/promoter DNA of Escherichia coli. Grunden, A.M., Self, W.T., Villain, M., Blalock, J.E., Shanmugam, K.T. J. Biol. Chem. (1999) [Pubmed]
  3. Molybdate transport and its effect on nitrogen utilization in the cyanobacterium Anabaena variabilis ATCC 29413. Zahalak, M., Pratte, B., Werth, K.J., Thiel, T. Mol. Microbiol. (2004) [Pubmed]
  4. Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. McNicholas, P.M., Rech, S.A., Gunsalus, R.P. Mol. Microbiol. (1997) [Pubmed]
  5. Functional dissection of the molybdate-responsive transcription regulator, ModE, from Escherichia coli. McNicholas, P.M., Mazzotta, M.M., Rech, S.A., Gunsalus, R.P. J. Bacteriol. (1998) [Pubmed]
  6. Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli. Rosentel, J.K., Healy, F., Maupin-Furlow, J.A., Lee, J.H., Shanmugam, K.T. J. Bacteriol. (1995) [Pubmed]
  7. Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene. Walkenhorst, H.M., Hemschemeier, S.K., Eichenlaub, R. Microbiol. Res. (1995) [Pubmed]
  8. Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability. Rech, S., Deppenmeier, U., Gunsalus, R.P. J. Bacteriol. (1995) [Pubmed]
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