Disease relevance of motB

• The motA and motB gene products of Escherichia coli are integral membrane proteins necessary for flagellar rotation [1].
• By placing motB under control of the tryptophan operon promoter of Serratia marcescens, we have succeeded in overproducing the MotB protein [2].

High impact information on motB

• This result confirms that translational coupling to motA is important for normal translation of the motB mRNA, since overproduction of MotA in trans did not improve complementation by pmotB [3].
• Mot protein assembly into the bacterial flagellum: a model based on mutational analysis of the motB gene [3].
• Class II suppressors were point mutations in the chromosomal motB or fliG genes [4].
• We have determined the nucleotide sequence of the motB region and find that it contains an open reading frame of 924 nucleotides which we ascribe to the motB gene [2].
• Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane [2].

Biological context of motB

• Sequence analysis revealed that the 1.6-kb transcript contains two open reading frames coding for protein sequences homologous to the Escherichia coli motA and motB gene products, respectively, and ends in a rho-independent termination site [5].
• A V. cholerae strain deleted of pomA, pomB, motX, and motY became weakly motile when the E. coli motA and motB genes were introduced on a plasmid [6].

Analytical, diagnostic and therapeutic context of motB

• To dissect the function of the periplasmic domain of MotB, site-directed mutagenesis was used to replace Gln, Ser, and Tyr codons scattered throughout motB with amber (UAG) codons [3].

References