Disease relevance of ampG

• The signal transducer encoded by ampG is essential for induction of chromosomal AmpC beta-lactamase in Escherichia coli by beta-lactam antibiotics and 'unspecific' inducers [1].

High impact information on ampG

• We have isolated mutants, containing alterations in a novel E. coli gene, ampG, in which a cloned C. freundii ampC gene is unable to respond to beta-lactam inducers [2].
• We have found that ampG and this ORF form an operon, whose promoter is located in front of the ORF [2].
• Immediately upstream of ampG there is another 579-base-pair-long open reading frame (ORF) encoding a putative lipoprotein shown to be non-essential for beta-lactamase induction [2].
• The ampG gene was cloned, sequenced and mapped to minute 9.6 on the E. coli chromosome [2].
• Transformation of both ampG and ampD into E. cloacae 55M-L reconstituted the inducible wild-type phenotype [3].

Biological context of ampG

• The nucleotide sequence of all four mutant ampG alleles (ampG1, ampG3, ampG4 and ampG5) was determined [1].
• These results suggested that the E. cloacae 55M-L phenotype was due to a mutation in an as yet unrecognized gene, designated ampG [3].
• Additionally it was shown that inactivation of the ampG-like gene irp8 did not affect either Fe-Ybt utilization or mouse virulence [4].

Analytical, diagnostic and therapeutic context of ampG

• An ampG gene was also detected in a Southern blot of DNA from the S. enteritidis isolate [5].

References