Disease relevance of ndh

• Regulation of transcription at the ndh promoter of Escherichia coli by FNR and novel factors [1].
• The structure of the multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes from cyanobacteria was investigated by growing the wild type and specific ndh His-tag mutants of Thermosynechococcus elongatus BP-1 under different CO(2) conditions, followed by an electron microscopy (EM) analysis of their purified membrane protein complexes [2].

High impact information on ndh

• Expression of the ndh-lacZ fusion in an fnr deletion strain was enhanced by anaerobic growth in rich medium or minimal medium supplemented with amino acids [1].
• Repression is not simply due to occlusion of the promoter (-35 and -10) region by FNR because adjacent pairs of FNR monomers were found to bind at two sites centred at -50.5 and -94.5 in the ndh promoter region without preventing RNA polymerase binding [1].
• Footprint studies indicated that FNR proteins lacking essential cysteine residues are impaired in their ability to protect FNR sites in the ndh promoter [3].
• The ndh gene regulates expression of the yjlC-ndh operon, as indicated by the fact that mutation in ndh causes a higher NADH/NAD(+) ratio [4].
• Expression of some operons, e.g., focA-pfl and fdnGHJI, was unaffected by reduction in the level of FNR, while the expression of other genes such as ndh and nikA was clearly affected [5].

Chemical compound and disease context of ndh

• The Escherichia coli ndh gene encodes NADH dehydrogenase II, a primary dehydrogenase used during aerobic and nitrate respiration [6].

Biological context of ndh

• Two putative binding sites for Arr, which overlap the two FNR boxes in the ndh promoter, were identified [7].
• A mutant strain complemented with ndh gene almost recovered the parental phenotype for growing in copper limitation or excess [8].
• NADH dehydrogenase-2 (NDH-2) from Escherichia coli respiratory chain is a membrane-bound cupric-reductase encoded by ndh gene [8].

Associations of ndh with chemical compounds

• Here, we report that the respiratory system of a ndh deficient strain suffered a faster inactivation than that of the parental strain in the presence of tert-butyl hydroperoxide due to endogenous copper [8].

Regulatory relationships of ndh

• Studies with an ndh-lacZ fusion confirmed that IHF represses ndh expression in vivo [7].

References