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Gene Review

ndh  -  NADH dehydrogenase

Escherichia coli CFT073

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Disease relevance of ndh


High impact information on ndh

  • These strains expressed binary combinations of one NADH dehydrogenase and one quinol oxidase [5].
  • Footprint studies indicated that FNR proteins lacking essential cysteine residues are impaired in their ability to protect FNR sites in the ndh promoter [6].
  • Expression of the fusion was found to be reduced during anaerobic growth, and experiments with hosts containing an fnr mutation and/or a multicopy fnr+ plasmid indicated that the anaerobic repression of the ndh gene is mediated by the FNR protein [2].
  • LPS induced a novel oxidant-dependent 3.8-kb mtDNA deletion in the region encoding NADH dehydrogenase subunits 1 and 2 and cytochrome c oxidase subunit I, which correlated with mitochondrial glutathione depletion [7].
  • In comparison, membranes from E. coli IY91, which contain amplified levels of NADH dehydrogenase, exhibit about 100-fold higher NADH/ubiquinone 1 reductase activity but about 20-fold less deamino-NADH/ubiquinone 1 reductase activity [8].

Chemical compound and disease context of ndh


Biological context of ndh

  • The ndh gene regulates expression of the yjlC-ndh operon, as indicated by the fact that mutation in ndh causes a higher NADH/NAD(+) ratio [3].
  • NADH dehydrogenase-2 (NDH-2) from Escherichia coli respiratory chain is a membrane-bound cupric-reductase encoded by ndh gene [10].
  • A mutant strain complemented with ndh gene almost recovered the parental phenotype for growing in copper limitation or excess [10].

Anatomical context of ndh


Associations of ndh with chemical compounds

  • Here, we report that the respiratory system of a ndh deficient strain suffered a faster inactivation than that of the parental strain in the presence of tert-butyl hydroperoxide due to endogenous copper [10].
  • The results indicated that only partial N-terminal processing was occurring in vitro and that the first residue of the unprocessed NADH dehydrogenase is N-formylmethionine [12].
  • Radiochemical N-terminal sequencing of the synthesized NADH dehydrogenase showed that the product was a mixture of three different species, with N-formylmethionine, methionine, or threonine at the N terminus [12].
  • One of these fragments, the soluble NADH dehydrogenase fragment, represents the electron input part of complex I. It comprises the subunits NuoE, F and G and harbors one flavin mononucleotide and up to six iron-sulfur clusters [13].
  • Thus, SDS-PAGE electrophoresis shows a gradual attenuation of some transport protein bands as the irradiation proceeds, while a complete loss of lactate and NADH dehydrogenase activities is caused by 15 min-exposure to light [14].

Analytical, diagnostic and therapeutic context of ndh


  1. Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803: identification of two new ndh gene products with nuclear-encoded homologues in the chloroplast Ndh complex. Prommeenate, P., Lennon, A.M., Markert, C., Hippler, M., Nixon, P.J. J. Biol. Chem. (2004) [Pubmed]
  2. FNR-dependent repression of the ndh gene of Escherichia coli and metal ion requirement for FNR-regulated gene expression. Spiro, S., Roberts, R.E., Guest, J.R. Mol. Microbiol. (1989) [Pubmed]
  3. Regulatory Loop between Redox Sensing of the NADH/NAD+ Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis. Gyan, S., Shiohira, Y., Sato, I., Takeuchi, M., Sato, T. J. Bacteriol. (2006) [Pubmed]
  4. A primary sodium pump gene of the moderate halophile Halobacillus dabanensis exhibits secondary antiporter properties. Yang, L., Jiang, J., Zhang, B., Zhao, B., Wang, L., Yang, S.S. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  5. Differentiation between electron transport sensing and proton motive force sensing by the Aer and Tsr receptors for aerotaxis. Edwards, J.C., Johnson, M.S., Taylor, B.L. Mol. Microbiol. (2006) [Pubmed]
  6. Properties of FNR proteins substituted at each of the five cysteine residues. Green, J., Sharrocks, A.D., Green, B., Geisow, M., Guest, J.R. Mol. Microbiol. (1993) [Pubmed]
  7. Postlipopolysaccharide oxidative damage of mitochondrial DNA. Suliman, H.B., Carraway, M.S., Piantadosi, C.A. Am. J. Respir. Crit. Care Med. (2003) [Pubmed]
  8. NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Matsushita, K., Ohnishi, T., Kaback, H.R. Biochemistry (1987) [Pubmed]
  9. Evidence for gene duplication forming similar binding folds for NAD(P)H and FAD in pyridine nucleotide-dependent flavoenzymes. McKie, J.H., Douglas, K.T. FEBS Lett. (1991) [Pubmed]
  10. The Cu(II)-reductase NADH dehydrogenase-2 of Escherichia coli improves the bacterial growth in extreme copper concentrations and increases the resistance to the damage caused by copper and hydroperoxide. Rodríguez-Montelongo, L., Volentini, S.I., Farías, R.N., Massa, E.M., Rapisarda, V.A. Arch. Biochem. Biophys. (2006) [Pubmed]
  11. Agrobacterium tumefaciens type II NADH dehydrogenase. Characterization and interactions with bacterial and thylakoid membranes. Bernard, L., Desplats, C., Mus, F., Cuiné, S., Cournac, L., Peltier, G. FEBS J. (2006) [Pubmed]
  12. In vitro synthesis of the respiratory NADH dehydrogenase of Escherichia coli. Role of UUG as initiation codon. Poulis, M.I., Shaw, D.C., Campbell, H.D., Young, I.G. Biochemistry (1981) [Pubmed]
  13. One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography. Bungert, S., Krafft, B., Schlesinger, R., Friedrich, T. FEBS Lett. (1999) [Pubmed]
  14. Photosensitization of wild and mutant strains of Escherichia coli by meso-tetra (N-methyl-4-pyridyl)porphine. Valduga, G., Breda, B., Giacometti, G.M., Jori, G., Reddi, E. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  15. Structural characterization of NDH-1 complexes of Thermosynechococcus elongatus by single particle electron microscopy. Arteni, A.A., Zhang, P., Battchikova, N., Ogawa, T., Aro, E.M., Boekema, E.J. Biochim. Biophys. Acta (2006) [Pubmed]
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