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Gene Review

purL  -  phosphoribosylformyl-glycineamide synthetase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2555, JW2541, purG, purI
 
 
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Disease relevance of purL

 

High impact information on purL

  • Escherichia coli 5'-phosphoribosylformylglycinamide (FGAR) amidotransferase (EC 6.3.5.3) encoded by the purL gene catalyzes the conversion of FGAR to formylglycinamidine in the presence of glutamine and ATP for the de novo purine nucleotide biosynthesis [2].
  • On the basis of the nucleotide sequence of purL, the enzyme was dissected along the polypeptide chain into at least three discrete regions, designated as domains I, II, and III, by genetic complementation tests [2].
  • The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced [4].
  • Comparison of the purL control region to other pur loci control regions reveals a common region of dyad symmetry which may be the binding site for the "putative" repressor protein [4].
  • These measurements indicated 5- to 17-fold coregulation of genes purF, purHD, purC, purMN, purL, and purEK and thus confirm the existence of a pur regulon [5].
 

Biological context of purL

  • A series of cold-sensitive mutations, affecting the assembly of ribosomes at 20 degrees C, was isolated within the purL to nadB region of the E. coli chromosome and one group, named rbaA, mapped at the same locus as the suppressor mutation, showing close linkage to the RNAase III gene [6].
  • In L. lactis there is a gene cluster, which contains five out of the 11 genes needed for the de novo biosynthesis of IMP, namely purC, orf, purQ, purL and purF [7].
 

Associations of purL with chemical compounds

  • Some of the mutants had acquired an additional genetic lesion in the purine de novo biosynthetic pathway, namely a purF, a purL or a purM mutation [8].

References

  1. Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei. Gu, Z.M., Martindale, D.W., Lee, B.H. Gene (1992) [Pubmed]
  2. The organization of the purL gene encoding 5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli. Sampei, G., Mizobuchi, K. J. Biol. Chem. (1989) [Pubmed]
  3. The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and characterization of the purC and purL genes. Jackson, M., Berthet, F.X., Otal, I., Rauzier, J., Martin, C., Gicquel, B., Guilhot, C. Microbiology (Reading, Engl.) (1996) [Pubmed]
  4. Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization. Schendel, F.J., Mueller, E., Stubbe, J., Shiau, A., Smith, J.M. Biochemistry (1989) [Pubmed]
  5. Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction. He, B., Shiau, A., Choi, K.Y., Zalkin, H., Smith, J.M. J. Bacteriol. (1990) [Pubmed]
  6. Suppressors of temperature-sensitive mutations in a ribosomal protein gene, rpsL (S12), of Escherichia coli K12. Nashimoto, H., Miura, A., Saito, H., Uchida, H. Mol. Gen. Genet. (1985) [Pubmed]
  7. Isolation and characterization of a purC(orf)QLF operon from Lactococcus [correction of Lactobacillus] lactis MG1614. Peltonen, T., Mäntsälä, P. Mol. Gen. Genet. (1999) [Pubmed]
  8. Role of guanosine kinase in the utilization of guanosine for nucleotide synthesis in Escherichia coli. Hove-Jensen, B., Nygaard, P. J. Gen. Microbiol. (1989) [Pubmed]
 
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