Disease relevance of galR

• The nucleotide sequence of the galR gene of Escherichia coli, which codes for galactose repressor, has been determined [1].

High impact information on galR

• To analyze the structure of the repressosome, we have screened and isolated galR mutants in which single amino acid substitutions in GalR lead to defects in loop formation while the protein's operator-binding activity is retained [2].
• The galR gene, which encodes the Gal repressor protein in Escherichia coli, has been fused to the strong pL promoter of bacteriophage lambda in plasmid pKC31 [3].
• The amino-terminal amino acid sequence determined by automated Edman degradation is: N-Ala-Thr-Ile-Lys-Asp-Val-Ala-Arg-Leu-Ala-Gly-Val-Ser-Val-Ala-Thr-Val-. Comparison of this sequence with that deduced from the DNA sequence of the galR gene showed that the formyl methionine residue preceding alanine at position 1 is cleaved off [3].
• The ascFB genes are paralogous to the cryptic bglFB genes, and ascG is paralogous to galR [4].
• Transcription from different promoters of the gal regulon, galP1, galP2 and mglP, was examined by primer extension and reverse transcription of mRNA isolated from strains containing mutations in galR and/or galS [5].

Biological context of galR

• At the 3' end of the lysA gene an open reading frame was revealed in the opposite direction and was identified as the galR coding region [6].
• Expression of a similar gene fusion of the galR promoter to lacZ was unregulated [7].
• In strains completely deleted for galR, the gene which encodes the Gal repressor, the operon is derepressed by only 10-fold without an inducer [8].
• The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts [7].
• Study of the promoter-lacZ gene fusions introduced into the chromosome indicated that galS expression was elevated in cells in which the normal galS gene was interrupted, but not in cells in which the galR gene was deleted [7].

References