Disease relevance of fepB

• Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli [1].

High impact information on fepB

• At the fepB operator, a 31 base-pair Fur-protected region was identified, corresponding to positions -19 to +12 with respect to the transcriptional start site [1].
• The fepB-entC promoter-operator elements were confirmed by mutation, using the dual gene fusion system in multicopy and low copy number states [1].
• In this study, we engineered a stable insertion mutation into the chromosomal region between the enterobactin genes fepB and entE [2].
• The nucleotide sequence of fepB and neighboring regions was determined [3].
• In vitro DNA-directed protein-synthesizing systems and experiments with the inhibitors dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and ethanol demonstrated that the initial fepB translation product is processed [3].

Biological context of fepB

• Control sequences directing iron-regulated expression of this polycistronic message were localized to the fepB-entC bidirectional promoter region [1].
• The upstream regulatory region included a Fur box, indicating that fepB is iron regulated, which was verified by RNA dot blot experiments [3].

Associations of fepB with chemical compounds

• An ORF of unknown significance was found downstream from fepB; its product would have a molecular weight of 18,036 and be rich in proline and alanine [3].

References