The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

entE  -  2,3-dihydroxybenzoate-AMP ligase component...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0587, JW0586
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of entE


High impact information on entE

  • These fragments were used as DNA/DNA hybridization probes to screen a wild-type gene library, yielding a recombinant cosmid (pEC7) able to complement mutations disrupting the 2,3-dihydroxybenzoic acid biosynthetic pathway in both Erwinia and Escherichia spp. as well as the E. coli entE mutation [3].
  • Reverse transcriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene [4].
  • In addition to its transcriptional activating function, AngR also complemented a mutation in the Escherichia coli entE gene, which encodes the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase [5].
  • Four closely linked open reading frames, corresponding to the coding regions of entE (carboxy-terminal 144 amino acids), entB (32,554 daltons), entA (26,249 daltons), and an unidentified gene (P15) encoding a 14,970-dalton protein, were found [6].
  • In this study, we engineered a stable insertion mutation into the chromosomal region between the enterobactin genes fepB and entE [7].

Biological context of entE

  • Analysis of various subclones and transposon insertion mutations confirmed the previously suggested gene order as entEBG(AC) and provided evidence to suggest that these genes are organized as three independent transcriptional units, composed of entE, entBG, and entAC, with the entBG mRNA transcribed in a clockwise direction [8].
  • In this work, an entE homologue, angE, was identified on pEIB1 (a pJM1-like plasmid) from virulent V. anguillarum serotype O1 strain MVM425 [2].
  • Nucleotide sequence of the Escherichia coli entE gene [9].

Associations of entE with chemical compounds

  • The E and F components were shown to catalyze ATP-pyrophosphate exchange reactions dependent on 2,3-dihydroxybenzoate and L-serine, respectively, whereas fractionated extracts of the entE and entF mutant strains lacked these reactions [1].


  1. Biosynthesis of enterochelin in Escherichia coli K-12: separation of the polypeptides coded for by the entD, E, F and G genes. Woodrow, G.C., Young, I.G., Gibson, F. Biochim. Biophys. Acta (1979) [Pubmed]
  2. Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1. Liu, Q., Ma, Y., Wu, H., Shao, M., Liu, H., Zhang, Y. Arch. Microbiol. (2004) [Pubmed]
  3. Genetic analysis of the Erwinia chrysanthemi 3937 chrysobactin iron-transport system: characterization of a gene cluster involved in uptake and biosynthetic pathways. Franza, T., Enard, C., van Gijsegem, F., Expert, D. Mol. Microbiol. (1991) [Pubmed]
  4. Genetic organization and iron-responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle. Bellaire, B.H., Elzer, P.H., Hagius, S., Walker, J., Baldwin, C.L., Roop, R.M. Infect. Immun. (2003) [Pubmed]
  5. A single amino acid change in AngR, a protein encoded by pJM1-like virulence plasmids, results in hyperproduction of anguibactin. Tolmasky, M.E., Actis, L.A., Crosa, J.H. Infect. Immun. (1993) [Pubmed]
  6. Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. Liu, J., Duncan, K., Walsh, C.T. J. Bacteriol. (1989) [Pubmed]
  7. Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes. Ozenberger, B.A., Brickman, T.J., McIntosh, M.A. J. Bacteriol. (1989) [Pubmed]
  8. Cluster of genes controlling synthesis and activation of 2,3-dihydroxybenzoic acid in production of enterobactin in Escherichia coli. Nahlik, M.S., Fleming, T.P., McIntosh, M.A. J. Bacteriol. (1987) [Pubmed]
  9. Nucleotide sequence of the Escherichia coli entE gene. Staab, J.F., Elkins, M.F., Earhart, C.F. FEMS Microbiol. Lett. (1989) [Pubmed]
WikiGenes - Universities