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Gene Review

entC  -  isochorismate synthase 1

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0586, JW0585, fepF
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Disease relevance of entC


High impact information on entC

  • The mutant protein was partially purified and shown to be devoid of 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity, whereas the entC gene product from strain AN191 exhibited normal isochorismate synthase function [5].
  • The gene products MbtB, MbtE and MbtF are proposed to be peptide synthetases, MbtC and MbtD polyketide synthases, MbtI an isochorismate synthase that provides a salicylate activated by MbtA, and MbtG a required hydroxylase [6].
  • Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli [7].
  • Similarly, Fur protected a 31 base-pair region in entC, corresponding to positions +1 to +31 in the message [7].
  • Control sequences directing iron-regulated expression of this polycistronic message were localized to the fepB-entC bidirectional promoter region [7].

Chemical compound and disease context of entC


Biological context of entC

  • Appropriate regulatory signals upstream of entC suggest that this operon is regulated by iron through interaction with the Fur repressor protein [1].
  • Finally, polarity effects from the insertion mutation in entC on downstream biosynthetic genes indicated that this locus is the promoter-proximal cistron in an ent operon comprising at least five genes [1].
  • The fepB-entC promoter-operator elements were confirmed by mutation, using the dual gene fusion system in multicopy and low copy number states [7].
  • The C-terminal portion of the deduced amino acid sequence showed 58% identity and 79% similarity with the E. coli EntC protein (isochorismate synthetase), the first enzyme in the E. coli 2,3-DHB biosynthetic pathway, suggesting that amoA probably encodes a step in 2,3-DHB biosynthesis and is the A. hydrophila equivalent of the E. coli entC gene [11].

Associations of entC with chemical compounds


Other interactions of entC


  1. Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes. Ozenberger, B.A., Brickman, T.J., McIntosh, M.A. J. Bacteriol. (1989) [Pubmed]
  2. Linkage between catecholate siderophores and the multicopper oxidase CueO in Escherichia coli. Grass, G., Thakali, K., Klebba, P.E., Thieme, D., Müller, A., Wildner, G.F., Rensing, C. J. Bacteriol. (2004) [Pubmed]
  3. Diversity of siderophore genes encoding biosynthesis of 2,3-dihydroxybenzoic acid in Aeromonas spp. Massad, G., Arceneaux, J.E., Byers, B.R. Biometals (1994) [Pubmed]
  4. Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin. Pelludat, C., Brem, D., Heesemann, J. J. Bacteriol. (2003) [Pubmed]
  5. The in vitro conversion of chorismate to isochorismate catalyzed by the Escherichia coli entC gene product. Evidence that EntA does not contribute to isochorismate synthase activity. Tummuru, M.K., Brickman, T.J., McIntosh, M.A. J. Biol. Chem. (1989) [Pubmed]
  6. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Quadri, L.E., Sello, J., Keating, T.A., Weinreb, P.H., Walsh, C.T. Chem. Biol. (1998) [Pubmed]
  7. Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli. Brickman, T.J., Ozenberger, B.A., McIntosh, M.A. J. Mol. Biol. (1990) [Pubmed]
  8. Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate. Liu, J., Quinn, N., Berchtold, G.A., Walsh, C.T. Biochemistry (1990) [Pubmed]
  9. Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa. Serino, L., Reimmann, C., Baur, H., Beyeler, M., Visca, P., Haas, D. Mol. Gen. Genet. (1995) [Pubmed]
  10. Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli. Daruwala, R., Bhattacharyya, D.K., Kwon, O., Meganathan, R. J. Bacteriol. (1997) [Pubmed]
  11. Cloning, mutagenesis, and nucleotide sequence of a siderophore biosynthetic gene (amoA) from Aeromonas hydrophila. Barghouthi, S., Payne, S.M., Arceneaux, J.E., Byers, B.R. J. Bacteriol. (1991) [Pubmed]
  12. An isochorismate hydroxymutase isogene in Escherichia coli. Müller, R., Dahm, C., Schulte, G., Leistner, E. FEBS Lett. (1996) [Pubmed]
  13. A new isochorismate synthase specifically involved in menaquinone (vitamin K2) biosynthesis encoded by the menF gene. Daruwala, R., Kwon, O., Meganathan, R., Hudspeth, M.E. FEMS Microbiol. Lett. (1996) [Pubmed]
  14. Clustering of isochorismate synthase genes menF and entC and channeling of isochorismate in Escherichia coli. Buss, K., Müller, R., Dahm, C., Gaitatzis, N., Skrzypczak-Pietraszek, E., Lohmann, S., Gassen, M., Leistner, E. Biochim. Biophys. Acta (2001) [Pubmed]
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