Disease relevance of xylF

• Molecular genetics of a receptor protein for D-xylose, encoded by the gene xylF, in Escherichia coli [1].
• Xylose uptake in Bacillus megaterium depends on expression of a putative H+/xylose symporter encoded by xylT, the last gene in the xyl operon [2].
• The xylT gene was functionally expressed in Lactobacillus plantarum 80, a strain which lacks proton motive force-linked D-xylose transport activity [3].

High impact information on xylF

• Expression of xylT is xylose inducible and subject to carbon catabolite repression mediated by CcpA and cre [2].
• The existence of a functional XylR was further suggested by the presence of xylO sequences upstream of xylA and xylT and by the requirement of D-xylose for the induction of D-xylose isomerase, D-xylulose kinase, and D-xylose transport activities in L. brevis [3].
• The DNA sequence of the 2.5 kb Bgl II fragment containing the xylF gene was then determined, which confirmed the above gene order and revealed two divergent operons, probably regulated in a co-ordinate manner, one containing enzymes for catabolism of D-xylose, and the other proteins comprising the XylFG receptor-transport system [1].

Analytical, diagnostic and therapeutic context of xylF

• Molecular cloning and functional expression in lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis [3].

References