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Gene Review

uhpT  -  hexose phosphate transporter

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3657, JW3641
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Disease relevance of uhpT

  • Expression of the Escherichia coli sugar phosphate transport system, encoded by the uhpT gene, is regulated by external glucose 6-phosphate through the action of three linked regulatory genes, uhpABC [1].

High impact information on uhpT

  • Gel electrophoretic mobility shift and DNase I and hydroxyl radical protection assays showed that UhpA bound specifically to the region of the uhpT promoter extending from -80 to -50 bp, relative to the transcription start site [2].
  • To examine the effect of protein phosphorylation on the binding of UhpA to target sequences in the uhpT promoter region, the UhpA protein was overexpressed and purified [2].
  • Studies with Escherichia coli cells showed that the transport systems encoded by glpT (sn-glycerol 3-phosphate transport) and uhpT (hexose phosphate transport) catalyze a reversible 32Pi:Pi exchange [3].
  • Deletion of even seven amino acids from the C-terminus resulted in complete loss of transcription activation at the uhpT promoter [4].
  • Footprinting experiments demonstrated that UhpA and CAP modestly stimulated each other's binding to the uhpT promoter, but did not affect the positioning of the binding sites [5].

Chemical compound and disease context of uhpT


Biological context of uhpT


Associations of uhpT with chemical compounds

  • Indeed, the use of an uhpT-lacZ fusion revealed that much higher concentrations of the inducer glucose 6-P were required to elevate uhpT transcription when the pho regulon was expressed [9].

Other interactions of uhpT

  • Comparison with the corrected sequence from E. coli revealed that the four uhp genes are closely spaced, with minimal intergenic distances, and that uhpC is nearly identical in length to uhpT, both of which have substantial sequence relatedness along their entire lengths [1].
  • The heterologous exchange showed a maximal velocity of 130 nmol/min/mg protein via the glpT system and 11 nmol/min/mg protein for the uhpT system [3].


  1. Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. Island, M.D., Wei, B.Y., Kadner, R.J. J. Bacteriol. (1992) [Pubmed]
  2. Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter. Dahl, J.L., Wei, B.Y., Kadner, R.J. J. Biol. Chem. (1997) [Pubmed]
  3. Reconstitution of sugar phosphate transport systems of Escherichia coli. Ambudkar, S.V., Larson, T.J., Maloney, P.C. J. Biol. Chem. (1986) [Pubmed]
  4. Action of receiver and activator modules of UhpA in transcriptional control of the Escherichia coli sugar phosphate transport system. Webber, C.A., Kadner, R.J. Mol. Microbiol. (1995) [Pubmed]
  5. Separate contributions of UhpA and CAP to activation of transcription of the uhpT promoter of Escherichia coli. Olekhnovich, I.N., Dahl, J.L., Kadner, R.J. J. Mol. Biol. (1999) [Pubmed]
  6. Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter. Olekhnovich, I.N., Kadner, R.J. J. Bacteriol. (2002) [Pubmed]
  7. Identification of two essential arginine residues in UhpT, the sugar phosphate antiporter of Escherichia coli. Fann, M., Davies, A.H., Varadhachary, A., Kuroda, T., Sevier, C., Tsuchiya, T., Maloney, P.C. J. Membr. Biol. (1998) [Pubmed]
  8. Topology of the Escherichia coli uhpT sugar-phosphate transporter analyzed by using TnphoA fusions. Lloyd, A.D., Kadner, R.J. J. Bacteriol. (1990) [Pubmed]
  9. Expression of the pho regulon interferes with induction of the uhpT gene in Escherichia coli K-12. Hoffer, S.M., van Uden, N., Tommassen, J. Arch. Microbiol. (2001) [Pubmed]
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