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Gene Review:

glpT - sn-glycerol-3-phosphate transporter

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2232, JW2234

Song, X.M. et al., Larson, T.J. et al., Schryvers, A. et al., Yang, B. et al., Ambudkar, S.V. et al., et al.

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Disease relevance of glpT

The promoter-proximal gene (glpT) of the glpT-glpQ operon of Escherichia coli encodes a membrane permease responsible for active transport of sn-glycerol 3-phosphate [1].
Glycerol-3-phosphate transport in Haemophilus influenzae: cloning, sequencing, and transcription analysis of the glpT gene [2].

High impact information on glpT

The heterologous exchange showed a maximal velocity of 130 nmol/min/mg protein via the glpT system and 11 nmol/min/mg protein for the uhpT system [3].
The substrates of the phosphodiesterase had no affinity for the glpT-encoded active transport system [1].
In addition, two integration host factor (IHF) binding sites were identified downstream of the glpT transcriptional start site at positions +15 to +51 and +193 to +227 [4].
The recombinant plasmid carrying glpT was able to complement the isogenic glpT mutant to wild-type levels of G3P uptake and permitted growth on a selective medium with G3P as a major carbon source [2].
The nucleotide sequences of the glpT gene were determined both directly from PCR products and from the cloned DNA insert of strain Eagan [2].

Biological context of glpT

The cell-free system yielded a second protein that is likely to be also coded for by the glpT operon [5].
The presence of a functional glpT gene in Haemophilus influenzae could be questioned, since there is only what appears to be a truncated glpT (HI0686, 143 nt in the 5'-end) available in the H. influenzae Rd genome database (Fleischmann et al. , 1995) [2].

Associations of glpT with chemical compounds

Glucose and other phosphotransferase system sugars inhibit Pi efflux by repressing glpT expression [6].
The expression of recombinant plasmids carrying the glpA gene (anaerobic glycerol-3-phosphate dehydrogenase) and the closely linked glpT gene (glycerol 3-phosphate transport) were studied under aerobic and anaerobic conditions [7].

Analytical, diagnostic and therapeutic context of glpT

A Northern blot analysis showed that the glpT genes in both Eagan and Rd strains were transcribed on a RNA of approximately 1.4 kb in size [2].

References

Periplasmic glycerophosphodiester phosphodiesterase of Escherichia coli, a new enzyme of the glp regulon. Larson, T.J., Ehrmann, M., Boos, W. J. Biol. Chem. (1983) [Pubmed]
Glycerol-3-phosphate transport in Haemophilus influenzae: cloning, sequencing, and transcription analysis of the glpT gene. Song, X.M., Forsgren, A., Janson, H. Gene (1998) [Pubmed]
Reconstitution of sugar phosphate transport systems of Escherichia coli. Ambudkar, S.V., Larson, T.J., Maloney, P.C. J. Biol. Chem. (1986) [Pubmed]
Action at a distance for glp repressor control of glpTQ transcription in Escherichia coli K-12. Yang, B., Gerhardt, S.G., Larson, T.J. Mol. Microbiol. (1997) [Pubmed]
Cell-free synthesis of proteins related to sn-glycerol-3-phosphate transport in Escherichia coli. Schumacher, G., Bussmann, K. J. Bacteriol. (1978) [Pubmed]
Pi exchange mediated by the GlpT-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. Elvin, C.M., Hardy, C.M., Rosenberg, H. J. Bacteriol. (1985) [Pubmed]
The anaerobic sn-glycerol-3-phosphate dehydrogenase: cloning and expression of the glpA gene of Escherichia coli and identification of the glpA products. Schryvers, A., Weiner, J.H. Can. J. Biochem. (1982) [Pubmed]

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