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Gene Review

fadR  -  fatty acid metabolism regulon...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1175, JW1176, dec, ole, oleR, ...
 
 
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Disease relevance of fadR

  • Fatty acid biosynthesis and fatty acid degradation in Escherichia coli are co-ordinately regulated at the level of transcription by the product of the fadR gene, FadR [1].
  • Recombinant Escherichia coli fadR atoC(Con) mutants containing the polyhydroxyalkanoate (PHA) biosynthesis genes from Alcaligenes eutrophus are able to incorporate significant levels of 3-hydroxyvalerate (3HV) into the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] [2].
  • An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F+ atoC2c atoD32 fadR) on butyrate as the sole source of carbon and energy [3].
 

High impact information on fadR

  • Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli [4].
  • Primer extension experiments demonstrated that the transcription of the fadR gene initiates with an adenine nucleotide 33 nucleotides upstream from the predicted start of translation [4].
  • The coding sequence of the fadR gene is 714 nucleotides long and is preceded by a typical E. coli ribosome binding site and is followed by a sequence predicted to be sufficient for factor-independent chain termination [4].
  • Thus, uspA is a member of the fadR regulon [5].
  • The growth phase-dependent increased and decreased transcription of fad genes and fabA, respectively, is dependent on the status of the fadR gene [5].
 

Biological context of fadR

  • We located the rap site at 26 min in the E. coli genetic map and determined the gene order fadR-rap-supF-trp from our transduction experiments [6].
 

Associations of fadR with chemical compounds

  • Transcription of uspA is derepressed during exponential growth in fadR null mutants or by including the fatty acid oleate in the growth medium of FadR+ cells [5].
 

Other interactions of fadR

  • Microarray data indicated that the expression levels of several important genes of acetate metabolism such as acs, aceAB, cysDEK, fadR, etc. were significantly altered in the absence of rpoS gene [7].
  • Similarly, E. coli fadR mutants were used alone and in conjunction with fadA, fadB, and fadL mutants to show that the effect of the fadR mutation is dependent on fadB+ and fadA+ gene products [2].
 

Analytical, diagnostic and therapeutic context of fadR

References

  1. Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichia coli by FadR. DiRusso, C.C., Metzger, A.K., Heimert, T.L. Mol. Microbiol. (1993) [Pubmed]
  2. Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli. Rhie, H.G., Dennis, D. Appl. Environ. Microbiol. (1995) [Pubmed]
  3. Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052. Oultram, J.D., Burr, I.D., Elmore, M.J., Minton, N.P. Gene (1993) [Pubmed]
  4. Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli. DiRusso, C.C. Nucleic Acids Res. (1988) [Pubmed]
  5. Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes. Farewell, A., Diez, A.A., DiRusso, C.C., Nyström, T. J. Bacteriol. (1996) [Pubmed]
  6. Genetic and physical location of the Escherichia coli rap locus, which is essential for growth of bacteriophage lambda. Guarneros, G., Machado, G., Guzmán, P., Garay, E. J. Bacteriol. (1987) [Pubmed]
  7. Effect of rpoS gene knockout on the metabolism of Escherichia coli during exponential growth phase and early stationary phase based on gene expressions, enzyme activities and intracellular metabolite concentrations. Rahman, M., Hasan, M.R., Oba, T., Shimizu, K. Biotechnol. Bioeng. (2006) [Pubmed]
  8. Characterization of the fatty acid-responsive transcription factor FadR. Biochemical and genetic analyses of the native conformation and functional domains. Raman, N., Black, P.N., DiRusso, C.C. J. Biol. Chem. (1997) [Pubmed]
 
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