Disease relevance of priB

• This suggests that priB and priC have a redundant and important role in Escherichia coli [1].

High impact information on priB

• The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification [2].
• A novel dnaC mutation that suppresses priB rep mutant phenotypes in Escherichia coli K-12 [3].
• This is supported herein by showing that like dnaC1331, a priB mutation is synthetically lethal with a dam mutation and this can be rescued by a mutH mutation [3].
• Furthermore, it is shown that dnaC824 will suppress phenotypes of priB priA300, rep priA300 and priB priC strains [3].
• The gain of function mutation dnaC809 restores the viability of holDG10 priA and holDG10 priB mutants only to some extent [4].

Biological context of priB

• DnaC809, a priA suppressor, failed to allow priA or priB mutants to grow using cSDR to initiate DNA replication [5].
• Like a priB mutation, a dnaC1331 mutation causes a priA2::kan-like phenotype when combined with priA300 [3].
• Isolation and characterization of a priB mutant of Escherichia coli influencing plasmid copy number of delta rop ColE1-type plasmids [6].
• The former contains the priB 3'-noncoding region and the reporter E. coli beta-galactosidase gene while the latter contains the priB 3'-noncoding region lacking the intron and the reporter gene [7].
• The putative polypeptides encoded by the open reading frames (ORFs) downstream from IRP3 and IRP4 were homologous, respectively, to several bacterial transcriptional regulators and to the deduced polypeptide encoded by an ORF located between the E. coli genes for primosomal replication protein N and adenine phosphoribosyltransferase [8].

Associations of priB with chemical compounds

• The 3'-noncoding region of the priB gene derived from the basidiomycete Lentinus edodes was found to contain one GC box-like sequence, two CAAT boxes, two TATA box-like sequences and two pyrimidine-rich stretches (CT-motifs) [7].

Other interactions of priB

• dnaC mutations suppress defects in DNA replication- and recombination-associated functions in priB and priC double mutants in Escherichia coli K-12 [1].

Analytical, diagnostic and therapeutic context of priB

• Southwestern blot analysis revealed that PRIB binds to the DNA fragment containing the upstream region of priB [9].

References