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Gene Review

rep  -  DNA helicase and single-stranded DNA...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3770, JW5604, dasC, mbrA
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Disease relevance of rep


High impact information on rep

  • We show that the RuvAB complex is required for the formation of a RusA substrate in the polymerase III mutants dnaEts and holD, affected for the Pol III catalytic subunit and clamp loader, and in the helicase mutant rep [4].
  • No increase in linear DNA was observed in the absence of replicating chromosomes, indicating that the formation of DSBs in rep strains requires replication [5].
  • Furthermore, it is shown that dnaC824 will suppress phenotypes of priB priA300, rep priA300 and priB priC strains [6].
  • Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD [1].
  • The org (rep) mutations confer amino acid substitutions in the viral coat protein, a constituent of the prohead, and the viral A protein, a constituent of the preinitiation complex [7].

Biological context of rep


Associations of rep with chemical compounds

  • The location of the promoter and the beginning of the protein were confirmed by S1 nuclease mapping of the 5' end of rep mRNA and determination of the NH2-terminal sequence of the rep protein [8].
  • Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without rep gene from plasmid pAG20 were constructed [11].

Other interactions of rep

  • Furthermore, they affect the growth of rep mutants, and to a lesser extent, that of recA mutants [12].


  1. Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD. Gilchrist, C.A., Denhardt, D.T. Nucleic Acids Res. (1987) [Pubmed]
  2. Ability of IncP-9 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions. Sevastsyanovich, Y.R., Titok, M.A., Krasowiak, R., Bingle, L.E., Thomas, C.M. Mol. Microbiol. (2005) [Pubmed]
  3. A novel replicon occurring naturally in Escherichia coli is a phage-plasmid hybrid. Seufert, W., Lurz, R., Messer, W. EMBO J. (1988) [Pubmed]
  4. RuvAB is essential for replication forks reversal in certain replication mutants. Baharoglu, Z., Petranovic, M., Flores, M.J., Michel, B. EMBO J. (2006) [Pubmed]
  5. DNA double-strand breaks caused by replication arrest. Michel, B., Ehrlich, S.D., Uzest, M. EMBO J. (1997) [Pubmed]
  6. A novel dnaC mutation that suppresses priB rep mutant phenotypes in Escherichia coli K-12. Boonsombat, R., Yeh, S.P., Milne, A., Sandler, S.J. Mol. Microbiol. (2006) [Pubmed]
  7. Host and phi X 174 mutations affecting the morphogenesis or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate. Ekechukwu, M.C., Oberste, D.J., Fane, B.A. Genetics (1995) [Pubmed]
  8. Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein. Bialkowska-Hobrzanska, H., Gilchrist, C.A., Denhardt, D.T. J. Bacteriol. (1985) [Pubmed]
  9. Synthesis of alpha3 phage DNA in replication mutants of Escherichia coli. Taketo, A., Kodaira, K.I. Biochim. Biophys. Acta (1978) [Pubmed]
  10. The Escherichia coli rep mutation. X. Consequences of increased and decreased Rep protein levels. Colasanti, J., Denhardt, D.T. Mol. Gen. Genet. (1987) [Pubmed]
  11. Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620. Kretová, M., Szemes, T., Laco, J., Gronesová, P., Grones, J. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  12. Viability of rep recA mutants depends on their capacity to cope with spontaneous oxidative damage and on the DnaK chaperone protein. Bredèche, M.F., Ehrlich, S.D., Michel, B. J. Bacteriol. (2001) [Pubmed]
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