The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Blotting, Southwestern

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Blotting, Southwestern


High impact information on Blotting, Southwestern


Biological context of Blotting, Southwestern

  • Activation of the reporter gene constructs appeared to be temporally and quantitatively correlated with the amount of nuclear C/EBP as determined by two-dimensional Western and Southwestern blot analyses [8].
  • When expressed from a cDNA expression vector as a fusion protein that also contained 115 kDa from beta-galactosidase, a Pnt2 binding protein (PCNBP) specifically bound to Pnt2 in Southwestern blots as a 30 kDa component of the 145 kDa fusion protein [9].

Anatomical context of Blotting, Southwestern

  • Gel retardation and Southwestern blot assays showed the presence of a 32-kD homeoprotein with DNA-binding properties typical of a HOX4 homeoprotein in nucleolar extracts of PHA-activated, but not of resting, lymphocytes [10].
  • In addition, Southwestern blot analysis detected binding of the most proximal Sp motif to a Sp1-like protein present in myoblast nuclear extracts but not in myotubes [11].
  • According to Southwestern blot analysis, a 94 kDa protein regarded as a GR was detected in F9 cells cultured in the medium containing the ginsenosides Rh1 or Rh2 [12].
  • Comparative Western and Southwestern blot analyses of nuclear extracts isolated from SMC and lung fibroblasts lay the foundation for possible differential regulation of elastin transcriptional levels via cell specific expression of different NF-1 family members [13].

Associations of Blotting, Southwestern with chemical compounds

  • This technique is complementary to gel retardation and Southwestern blotting analyses that have been previously used to identify cellular components that specifically bind to cisplatin-damaged DNA [14].
  • Southwestern blotting and UV-cross-linking studies showed that the HS3D probe recognized a approximately 35-kDa nuclear protein, and antibody supershift assays indicated that C/EBPdelta comprised most of the PGE2-activated gel-shifted complex [15].
  • Dexamethasone inhibits the phenobarbitone-mediated increase in the binding of a transcription factor(s) to the upstream region of the gene as evidenced by gel retardation and Southwestern blot analysis [16].
  • Southwestern blotting revealed four proteins from progesterone-dominated endometrial nuclei that bind UG200 [17].
  • By Southwestern blot analyses in which proteins were initially separated on a denaturing sodium dodecyl sulfate-gel, transferred to a membrane, and then probed with radio-labeled DNA, several different proteins were bound by either the palindromic or the variant CRE sequences [18].

Gene context of Blotting, Southwestern

  • UV cross-linking and Southwestern blot analyses revealed multiple DNA-protein interactions of which approximately 100- and approximately 45-kDa proteins were predominant; the approximately 45-kDa protein may represent CREB [19].
  • Subfragments of the region from -490 to -310 of the alpha-subunit promoter were used in a Southwestern blot assay using bacterially produced Msx1 and demonstrated that binding was localized specifically to the region from -449 to -421 [20].
  • On southwestern blots, a 40 kDa nuclear protein from C7-10 cells bound to DNA containing AP-1 sites [21].
  • Southwestern blot experiments demonstrate that, like the Ku protein, the smaller YPF1 subunit binds DNA in the absence of the larger subunit [22].
  • Southwestern blot analysis revealed that the labelled ANG nt -806/-779 interacted with two mouse liver nuclear proteins with apparent molecular masses of 52 and 43 kDa, whereas the labelled SOM-CRE, TAT-CRE and the CRE of the phosphoenolpyruvate carboxykinase (PEPCK) gene interacted with one molecular species of 43 kDa [23].


  1. Protein kinase A-dependent derepression of the human prodynorphin gene via differential binding to an intragenic silencer element. Carrión, A.M., Mellström, B., Naranjo, J.R. Mol. Cell. Biol. (1998) [Pubmed]
  2. Binding of a protein-tyrosine phosphatase to DNA through its carboxy-terminal noncatalytic domain. Radha, V., Kamatkar, S., Swarup, G. Biochemistry (1993) [Pubmed]
  3. Centromere protein B assembles human centromeric alpha-satellite DNA at the 17-bp sequence, CENP-B box. Muro, Y., Masumoto, H., Yoda, K., Nozaki, N., Ohashi, M., Okazaki, T. J. Cell Biol. (1992) [Pubmed]
  4. DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences. Ariga, H., Imamura, Y., Iguchi-Ariga, S.M. EMBO J. (1989) [Pubmed]
  5. Heterogeneous nuclear ribonucleoprotein (hnRNP) binding to hormone response elements: a cause of vitamin D resistance. Chen, H., Hewison, M., Hu, B., Adams, J.S. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  6. Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae. Schmitt, A.P., McEntee, K. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  7. Characterization of a nuclear protein that binds to three elements within the silencer region of a bean chalcone synthase gene promoter. Harrison, M.J., Lawton, M.A., Lamb, C.J., Dixon, R.A. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  8. Role of CAAT-enhancer binding protein isoforms in the cytokine regulation of acute-phase plasma protein genes. Baumann, H., Morella, K.K., Campos, S.P., Cao, Z., Jahreis, G.P. J. Biol. Chem. (1992) [Pubmed]
  9. Identification and characterization of a JC virus pentanucleotide repeat element binding protein: cellular nucleic acid binding protein. Liu, M., Kumar, K.U., Pater, M.M., Pater, A. Virus Res. (1998) [Pubmed]
  10. Expression of HOXC4 homeoprotein in the nucleus of activated human lymphocytes. Meazza, R., Faiella, A., Corsetti, M.T., Airoldi, I., Ferrini, S., Boncinelli, E., Corte, G. Blood (1995) [Pubmed]
  11. Sp1- and Sp3-mediated transcriptional regulation of the fibroblast growth factor receptor 1 gene in chicken skeletal muscle cells. Parakati, R., DiMario, J.X. J. Biol. Chem. (2002) [Pubmed]
  12. In vitro induction of differentiation by ginsenoides in F9 teratocarcinoma cells. Lee, Y.N., Lee, H.Y., Chung, H.Y., Kim, S.I., Lee, S.K., Park, B.C., Kim, K.W. Eur. J. Cancer (1996) [Pubmed]
  13. The role of NF-1 factors in regulation of elastin gene transcription. Degterev, A., Foster, J.A. Matrix Biol. (1999) [Pubmed]
  14. Purification of nuclear proteins that bind to cisplatin-damaged DNA. Identity with high mobility group proteins 1 and 2. Hughes, E.N., Engelsberg, B.N., Billings, P.C. J. Biol. Chem. (1992) [Pubmed]
  15. CCAAT/enhancer-binding protein delta activates insulin-like growth factor-I gene transcription in osteoblasts. Identification of a novel cyclic AMP signaling pathway in bone. Umayahara, Y., Ji, C., Centrella, M., Rotwein, P., McCarthy, T.L. J. Biol. Chem. (1997) [Pubmed]
  16. Dexamethasone negatively regulates phenobarbitone-activated transcription but synergistically enhances cytoplasmic levels of cytochrome P-450b/e messenger RNA. Rao, M.V., Rangarajan, P.N., Padmanaban, G. J. Biol. Chem. (1990) [Pubmed]
  17. Prolactin augments progesterone-dependent uteroglobin gene expression by modulating promoter-binding proteins. Kleis-SanFrancisco, S., Hewetson, A., Chilton, B.S. Mol. Endocrinol. (1993) [Pubmed]
  18. Binding specificity of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins and activating transcription factors to naturally occurring CRE sequence variants. Drust, D.S., Troccoli, N.M., Jameson, J.L. Mol. Endocrinol. (1991) [Pubmed]
  19. Two CGTCA motifs and a GHF1/Pit1 binding site mediate cAMP-dependent protein kinase A regulation of human growth hormone gene expression in rat anterior pituitary GC cells. Shepard, A.R., Zhang, W., Eberhardt, N.L. J. Biol. Chem. (1994) [Pubmed]
  20. Msx1 is present in thyrotropic cells and binds to a consensus site on the glycoprotein hormone alpha-subunit promoter. Sarapura, V.D., Strouth, H.L., Gordon, D.F., Wood, W.M., Ridgway, E.C. Mol. Endocrinol. (1997) [Pubmed]
  21. Decreased survival of mosquito cells after stable transfection with a Drosophila ecdysteroid response element: possible involvement of a 40 kDa DNA binding protein. Jayachandran, G., Fallon, A.M. J. Insect Sci. (2002) [Pubmed]
  22. Yolk protein factor 1 is a Drosophila homolog of Ku, the DNA-binding subunit of a DNA-dependent protein kinase from humans. Jacoby, D.B., Wensink, P.C. J. Biol. Chem. (1994) [Pubmed]
  23. Identification of a novel mouse hepatic 52 kDa protein that interacts with the cAMP response element of the rat angiotensinogen gene. Wu, J., Jiang, Q., Chen, X., Wu, X.H., Chan, J.S. Biochem. J. (1998) [Pubmed]
WikiGenes - Universities