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Gene Review:

nifB - nitrogen fixation protein NifB

Sinorhizobium fredii NGR234

Masepohl, B. et al., Rossen, L. et al., Joerger, R.D. et al., Grönger, P. et al., Souza, E.M. et al., et al.

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Disease relevance of nifB

Identification and DNA sequence of fixZ, a nifB-like gene from Rhizobium leguminosarum [1].
Two of these alleles, fix52::Tn5 and fix137::Tn5 were in a region of pRL 1JI which hybridized to a probe that contained the nifA gene and the amino-terminal region of the nifB gene of Klebsiella pneumoniae [1].
Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii [2].
Sequence and structural organization of a nif A-like gene and part of a nifB-like gene of Herbaspirillum seropedicae strain Z78 [3].

High impact information on nifB

One of these loci corresponded to nifA and the other to nifB [4].
The ORF downstream from nifB predicts an amino acid sequence with a cysteine residue pattern that is characteristic of ferredoxins [2].
Plasmid integration mutagenesis additionally revealed that nifA, nifB and fdxN form one transcriptional unit [5].
A rho-independent termination signal and a typical nif promoter region containing a putative NifA binding site and a consensus nif promoter are located within the region between the R. capsulatus nifA and nifB genes [6].
Two open reading frames coding for a 59,653 (NifA) and a 49,453 (NifB) dalton protein could be detected [6].

Biological context of nifB

Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame [7].

References

Identification and DNA sequence of fixZ, a nifB-like gene from Rhizobium leguminosarum. Rossen, L., Ma, Q.S., Mudd, E.A., Johnston, A.W., Downie, J.A. Nucleic Acids Res. (1984) [Pubmed]
Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii. Joerger, R.D., Bishop, P.E. J. Bacteriol. (1988) [Pubmed]
Sequence and structural organization of a nif A-like gene and part of a nifB-like gene of Herbaspirillum seropedicae strain Z78. Souza, E.M., Funayama, S., Rigo, L.U., Yates, M.G., Pedrosa, F.O. J. Gen. Microbiol. (1991) [Pubmed]
Identification of a nifA-like regulatory gene of Azospirillum brasilense Sp7 expressed under conditions of nitrogen fixation and in the presence of air and ammonia. Liang, Y.Y., Kaminski, P.A., Elmerich, C. Mol. Microbiol. (1991) [Pubmed]
The Rhizobium meliloti fdxN gene encoding a ferredoxin-like protein is necessary for nitrogen fixation and is cotranscribed with nifA and nifB. Klipp, W., Reiländer, H., Schlüter, A., Krey, R., Pühler, A. Mol. Gen. Genet. (1989) [Pubmed]
Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus. Masepohl, B., Klipp, W., Pühler, A. Mol. Gen. Genet. (1988) [Pubmed]
Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Grönger, P., Manian, S.S., Reiländer, H., O'Connell, M., Priefer, U.B., Pühler, A. Nucleic Acids Res. (1987) [Pubmed]

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