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Gene Review

TGS1  -  trimethylguanosine synthase 1

Homo sapiens

Synonyms: CLL-associated antigen KW-2, Cap-specific guanine-N2 methyltransferase, HCA137, Hepatocellular carcinoma-associated antigen 137, NCOA6IP, ...
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Disease relevance of TGS1

  • Here we investigated PIMT regulation in astrocytic tumors, which are the most common human brain tumors [1].
  • More precisely, PIMT levels were significantly lower by 76% in pilocytic astrocytomas (grade I), 46% in astrocytomas (grade II), 69% in anaplastic astrocytomas (grade III), and a marked 80% in glioblastomas (grade IV) as compared to normal brains [1].
  • Similar results were obtained, either by measuring the reduction in PIMT activity and expression or by measuring the formation of abnormal proteins, in an orthotopic rat brain tumor model implanted with invasive CNS-1 glioma cells [1].
  • In this study, a promising PGA gene from Alcaligenes faecalis (afpga) and another pcm gene encoding protein isoaspartate methyltransferase (PIMT) were constructed into pET43.1a((+)) and pET28a((+)), respectively [2].

High impact information on TGS1

  • In contrast, PIMT functioned as a repressor of CBP/p300-mediated transactivation of PPARgamma [3].
  • The N-terminal of PIMT (residues 1-369) containing the RNA binding domain interacts with both C/H1 and C/H3 domains of CBP and p300 and with the C-terminal portion of PBP that encompasses amino acids 1371-1560 [3].
  • RT-PCR analysis showed that levels of type I PIMT mRNA were up-regulated while those of type II PIMT mRNA were down-regulated in glioblastomas [1].
  • We previously reported that PIMT expression and activity are reduced by half in human epileptic hippocampus [1].
  • Inhibition of PIMT/Tgs1 expression by siRNA in HeLa cells resulted in an increase in the percentage of cells in G2/M phases [4].

Associations of TGS1 with chemical compounds

  • PIMT increased the enzymatic activities in supernatant of ferment broth (1.6 folds) and cell lysate (1.8 folds), while it did not significantly affect the expression level of penicillin G acylase [2].


  1. Expression and activity of l-isoaspartyl methyltransferase decrease in stage progression of human astrocytic tumors. Lapointe, M., Lanthier, J., Moumdjian, R., Régina, A., Desrosiers, R.R. Brain Res. Mol. Brain Res. (2005) [Pubmed]
  2. Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene. Wang, T., Zhu, H., Ma, X., Fei, Z., Ma, Y., Wei, D. Appl. Microbiol. Biotechnol. (2006) [Pubmed]
  3. Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation. Misra, P., Qi, C., Yu, S., Shah, S.H., Cao, W.Q., Rao, M.S., Thimmapaya, B., Zhu, Y., Reddy, J.K. J. Biol. Chem. (2002) [Pubmed]
  4. Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus. Enünlü, I., Pápai, G., Cserpán, I., Udvardy, A., Jeang, K.T., Boros, I. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
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