Disease relevance of Torovirus

• Surprisingly, in contrast to the M(pro)s of corona- and roniviruses, but like that of arterivirus, the torovirus M(pro) uses serine instead of cysteine as its principal nucleophile [1].

High impact information on Torovirus

• Sequence analysis of the 3' part (8 kb) of the polymerase gene of the torovirus prototype Berne virus (BEV) revealed that this area contains at least two open reading frames (provisionally designated ORF1a and ORF1b) which overlap by 12 nucleotides [2].
• Comparative sequence analysis of polyprotein 1a of equine torovirus (EToV) strain Berne, identified a serine proteinase domain, flanked by hydrophobic regions [1].
• Hemagglutinin-esterase, a novel structural protein of torovirus [3].
• Rabbits were inoculated with material from the gradient peak fractions of human stool specimens, and their sera were assayed for immunologic comparison with bovine torovirus as a reference [4].
• The replication of Berne virus was reduced more than 1000-fold by actinomycin D, when the drug (0.1 to 1.0 micrograms/ml) was added during the first 8 h after infection; alpha-amanitin (25 micrograms/ml) produced a similar though less pronounced effect [5].

Chemical compound and disease context of Torovirus

• One of the viruses had a density in sucrose solution of 1.16, similar to the value for Berne virus [6].

Biological context of Torovirus

• We cloned and sequenced amplification products obtained by low-stringency polymerase chain reaction amplification using consensus primers mapping to the 3' end of the genome of animal toroviruses, but found no significant homologies with animals torovirus sequences [4].
• The serotypes 1 and 2 of Breda virus from feces of experimentally infected gnotobiotic calves were studied with respect to their sedimentation and density properties in sucrose gradients and their structural polypeptides; Berne virus, the proposed prototype of the new family Toroviridae, was included for comparison [7].

Analytical, diagnostic and therapeutic context of Torovirus

• By immunoelectron microscopy (IEM) torovirus particles from BTV-positive specimens from calves with diarrhea and HTV-positive specimens from patients were aggregated by the hyperimmune sera [8].

References