Disease relevance of Immunosorbent Techniques

• Concentrations of interleukin-2 receptors were studied in sera of 81 patients with pauciarticular-, polyarticular-, and systemic-onset juvenile chronic arthritis using a double antibody "sandwich" enzyme-linked immunosorbent technique [1].

High impact information on Immunosorbent Techniques

• We have used an immunoadsorbent technique to extract and concentrate hGH from large plasma samples before RIA in order to precisely measure basal hGH levels [immunoextracted RIA (IERIA)] [2].
• An amphiphilic detergent-solubilized human adult intestinal microvillous aminopeptidase N (EC 3.4.11.2) was purified by immunoadsorbent technique using meconial aminopeptidase N antibodies [3].
• These sera were assayed for IgM antibody detection by sucrose density gradient (SDG), the commercial ELISAs (Enzygnost IgM and Rubazyme M), and the non-commercial anti-my-hemadsorption immunosorbent technique (HIT) [4].
• An enzyme-linked immunosorbent technique for human serum retinol-binding protein (RBP) was developed [5].
• This method of preparation of human beta 2-microglobulin-associated membrane antigens has several advantages over classical immunoadsorbent techniques [6].

Associations of Immunosorbent Techniques with chemical compounds

• Human coagulation factor IX was purified by two ion-exchange chromatographies on DEAE-Sephadex A-50, heparin-Sepharose chromatography, hydroxyapatite chromatography and immunoadsorbent technique [7].
• We measured anticardiolipin antibodies (aCLs), antiphosphatidylserine antibodies (aPSs), anti-beta(2) glycoprotein I antibodies (anti-beta(2)GPIs), and antiprothrombin antibodies (aPTs) with an enzyme-linked immunosorbent technique using "in-house" assays [8].
• M0s in culture were exposed to iron as either ferric ammonium citrate or iron dextran, and ferritin synthesis was determined by measurement of 3H-leucine incorporation after isolation of ferritin by a quantitative immunoadsorbent technique [9].

Gene context of Immunosorbent Techniques

• We used an immunoadsorbent technique to extract hGH from large plasma samples to precisely measure basal hGH concentrations and their variation with time [10].
• As revealed by a preparative immunosorbent technique combined with isotype quantitation the majority of IgG2a- or IgG1-bound immunoglobulins isolated from the serum of virus-infected mice belonged to the same subclass as the target antibody [11].
• The pregnancy-specific beta 1-glycoprotein, SP1, was purified to homogeneity from placental extract and from serum using immunoadsorbent techniques [12].

References