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Kopp, J. et al.

Kopp, Wang, Kulmburg, Schultze-Mosgau, Huan, Ying, Seyhan, Jeschke, Kneser, Bach, Ge, Dooley, Horch,

Accelerated wound healing by in vivo application of keratinocytes overexpressing KGF.

Epidermal regeneration is a complex process, strongly influenced by growth factors, including keratinocyte growth factor (KGF). The objective of this study was to establish immortalized HaCaT keratinocytes and KMST-6-fibroblasts stably expressing KGF. Transfection efficiency, genomic integration, and functionality of the transgene were determined by ELISA and PCR, and KGF-expressing clones were selected using an air-liquid interface test system. HaCaT cells displayed stronger transgene expression compared to transfected fibroblasts, and the most effective HaCaT clone was incubated on a membrane carrier to form a "membrane cell graft." Twenty-one superficial second-degree burn wounds were created in each of three pigs, and wound healing capacity of the generated "polypeptide cell delivery system" after grafting was examined. Untransfected HaCaT keratinocytes and membrane-covered and untreated burn wounds served as controls. Histological and macroscopical follow-up revealed that grafting of transfected HaCaT cells resulted in complete reepithelialization within 5 days, while wounds covered with untransfected cells needed 2 days longer. At untreated sites, a thin epithelium was detectable after 10 days. The results indicate that wound healing processes can be stimulated distinctly by growth factors secreted from HaCaT cells, with a prominent role for transgenic KGF.[1]

References

Accelerated wound healing by in vivo application of keratinocytes overexpressing KGF. Kopp, J., Wang, G.Y., Kulmburg, P., Schultze-Mosgau, S., Huan, J.N., Ying, K., Seyhan, H., Jeschke, M.D., Kneser, U., Bach, A.D., Ge, S.D., Dooley, S., Horch, R.E. Mol. Ther. (2004) [Pubmed]

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