Disease relevance of FGF7

• The pattern of epidermal thickening observed in KGF-treated wounds resembled psoriasis [1].

High impact information on FGF7

• Human keratinocyte growth factor effects in a porcine model of epidermal wound healing [1].
• Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor (FGF) family (hence the alternative designation FGF-7) [1].
• KGF treatment also increased the number of serrated basal cells associated with increased deposition of collagen fibers in the superficial dermis adjacent to the acanthotic epidermis [1].
• In these cells, MMP-13 expression at the mRNA and protein level was potently enhanced (up to sixfold) by tumor necrosis factor-alpha, transforming growth factor-beta(1), and transforming growth factor-alpha and by keratinocyte growth factor in the presence of heparin [2].
• Growth factors in porcine full and partial thickness burn repair. Differing targets and effects of keratinocyte growth factor, platelet-derived growth factor-BB, epidermal growth factor, and neu differentiation factor [3].

Biological context of FGF7

• Genetic linkage mapping of the porcine fibroblast growth factor 7 (FGF7) gene [4].
• Transfection efficiency, genomic integration, and functionality of the transgene were determined by ELISA and PCR, and KGF-expressing clones were selected using an air-liquid interface test system [5].
• To determine the factors affecting KGF expression in the uterus, endometrial explants from gilts on day 9 of the estrous cycle were cultured in the presence of 17beta-estradiol, catechol estrogens, or progesterone [6].
• Colocalization of heparin and receptor binding sites on keratinocyte growth factor [7].
• Keratinocyte growth factor is up-regulated by estrogen in the porcine uterine endometrium and functions in trophectoderm cell proliferation and differentiation [6].

Anatomical context of FGF7

• Keratinocyte growth factor/fibroblast growth factor-7 (KGF/FGF-7) is an established paracrine mediator of hormone-regulated epithelial growth and differentiation [8].
• Keratinocyte growth factor: expression by endometrial epithelia of the porcine uterus [8].
• DESIGN: Freshly isolated porcine tracheal epithelial cells were cytospun onto glass slides for immunohistochemical identification and localization of KGF and for in situ hybridization localization of its messenger RNA [9].
• Keratinocyte growth factor and autocrine repair in airway epithelium [9].
• The objective of this study was to establish immortalized HaCaT keratinocytes and KMST-6-fibroblasts stably expressing KGF [5].

Associations of FGF7 with chemical compounds

• 17beta-Estradiol stimulated the expression of KGF (P < 0.05), whereas catechol estrogens had no effect (P > 0.05) [6].
• To identify regions in KGF that contribute to heparin and tyrosine kinase receptor interactions, nine peptides spanning defined motifs in the predicted structure of KGF were synthesized, and their heparin and receptor binding properties were analyzed [7].
• Heparin and heparan sulfate in combination with KGF may thus have important roles in the regulation of epithelial cell collagenase under conditions such as inflammation and wound healing [10].
• In the operated ureter elevated expression of keratinocyte growth factor, vascular endothelial growth factor, TGF-alpha, TGF-beta 1, TGF-beta 3 and integrin was detected 2 hours after the operation and sustained for 7 to 14 days after the procedure [11].
• CLA and n-3 PUFA acted synergistically to upregulate colonic KGF expression in DSS-challenged pigs but n-3 PUFA blocked CLA-induced PPAR gamma activation [12].

Other interactions of FGF7

• The colonic expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma), PPAR gamma- and delta-responsive genes, keratinocyte growth factor (KGF) and tumor necrosis factor (TNF-alpha) were assayed by quantitative RT-PCR [12].
• Sequence of keratinocyte growth factor and keratinocyte growth factor receptor orthologs [monkey, pig, rabbit] [13].
• The primary tissue monolayer was plated, yielding secondary monolayers that were supplemented in culture with the following factors: insulin transferrin selenium, niacinamide, keratinocyte growth factor, and high glucose, which promoted formation of islet cell-like clusters during 30 days of culture [14].

Analytical, diagnostic and therapeutic context of FGF7

• Polymerase chain reaction analysis for KGF was also performed [9].
• Immunohistochemical analysis and in situ hybridization revealed a subset of cells that were positive for both the protein and the message for KGF [9].
• The topical application of recombinant growth factors such as epidermal growth factor, platelet-derived growth factor-BB homodimer (rPDGF-BB), keratinocyte growth factor (rKGF), and neu differentiation factor has resulted in significant acceleration of healing in several animal models of wound repair [3].
• Immunocytochemistry showed KGF predominantly in theca and small luteal cells [15].

References