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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Comparative physical localization of maize mir1 gene in Zea mays L. and Coix lacryma-jobi L.

The maize gene mir1 encoded a cystein proteinase which is resistant to fall armyworm. Previously, RFLP map indicated that the mir1 was mapped on chromosome 6. However, physical location of the mir1 gene on chromosome 6 has not been reported. In this study, the mir1 gene was physically located on the short arm of metaphase and pachytene chromosome 6 by dual-color fluorescence in situ hybridization (FISH) with 45S rDNA as a reference marker. The results of Southern blotting suggested that there were sequences homologous to mir1 in Coix lacryma-jobi L. genome. Then, the sequences were mapped on the distal region of long arm of chromosome 7 in C. lacryma-jobi by FISH. The percent distance from the signal site to centromere was 73.33 +/- 0.15.[1]

References

  1. Comparative physical localization of maize mir1 gene in Zea mays L. and Coix lacryma-jobi L. Han, Y.H., Wang, X.L., Liu, L.H., Song, Y.C. Yi Chuan Xue Bao (2004) [Pubmed]
 
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