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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Flow cytometric analysis of the in situ hybridization of cyclooxygenase isoforms in mesangial cells treated with cyclosporine A.

BACKGROUND: Cyclosporine A increases oxidative stress in kidney and we hypothesized that cyclooxygenase (COX) may be involved in this effect. MATERIAL AND METHODS: Mesangial cells of Cyclosporine A-treated (4, 7 or 10 days) rats were obtained to evaluate mRNA expression of COX-isoforms ( COX-1, constitutive and COX-2, inducible) by "in situ" hybridization. Probes were labelled using "Gene Image Random Prime Labelling Protocol" and COX expression was measured by flow cytometry. RESULTS AND DISCUSSION: "In situ" hybridization by flow cytometry is an useful method to detect mRNA. We observed an increased COX-2 expression in a time-dependent manner in parallel with Reactive Oxygen Species synthesis. COX-1 expression increased only at 10 days.[1]

References

  1. Flow cytometric analysis of the in situ hybridization of cyclooxygenase isoforms in mesangial cells treated with cyclosporine A. Pérez de Hornedo, J., Calvino Fernández, M., de Arriba de la Fuente, G., del Reino Fernández, P., Parra Cid, T., Benito Martinez, S. Cytometry. Part A : the journal of the International Society for Analytical Cytology. (2006) [Pubmed]
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