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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development.

The activity of hypoxanthine phosphoribosyltransferase (HPRT) was determined in the bovine embryo during early embryonic development. Microassay, using [(3)H] hypoxanthine, was improved to measure enzyme activity in the embryonic extract. This activity depended on the reaction time and the concentration of phosphorybosyl pyrophosphate (PRPP) in a reaction. mixture. Maximum activity was obtained at 4 hours of reaction time and at a concentration of 1 mM PRPP, but was much lower than the activity recorded in the mouse embryo. During early embryonic development, HPRT activity rapidly increased beyond the 8-cell stage. When distributions and activities of HPRT, adenine phosphorybosyltransferase (APRT), and the ratio of HPRT: APRT were examined in individual blastocysts, HPRT activity was broadly distributed, but it did not clearly show the bimodal distribution expected. Six of demi-embryos with high or low HPRT:APRT ratios were transferred to recipient cows from which 2 calves were obtained. Both offspring were of the sex predicted by the HPRT: APRT ratio. These results indicate that HPRT activity of bovine preimplantation embryos can be microassayed using radiolabeled hypoxanthine, and this assay could provide an alternative method for embryo sexing.[1]


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